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The Cluster Transfer Function of AtNEET Supports the Ferredoxin–Thioredoxin Network of Plant Cells
dc.contributor.author | I Zandalinas, Sara | |
dc.contributor.author | Song, Luhua | |
dc.contributor.author | Nechushtai, Rachel | |
dc.contributor.author | Mendoza-Cozatl, David G. | |
dc.contributor.author | Mittler, Ron | |
dc.date.accessioned | 2022-10-14T07:37:25Z | |
dc.date.available | 2022-10-14T07:37:25Z | |
dc.date.issued | 2022-08 | |
dc.identifier.citation | Zandalinas, S.I.; Song, L.; Nechushtai, R.; Mendoza-Cozatl, D.G.; Mittler, R. The Cluster Transfer Function of AtNEET Supports the Ferredoxin–Thioredoxin Network of Plant Cells. Antioxidants 2022, 11, 1533. https://doi.org/10.3390/antiox11081533 | ca_CA |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | http://hdl.handle.net/10234/200376 | |
dc.description.abstract | NEET proteins are conserved 2Fe-2S proteins that regulate the levels of iron and reactive oxygen species in plant and mammalian cells. Previous studies of seedlings with constitutive expression of AtNEET, or its dominant-negative variant H89C (impaired in 2Fe-2S cluster transfer), revealed that disrupting AtNEET function causes oxidative stress, chloroplast iron overload, activation of iron-deficiency responses, and cell death. Because disrupting AtNEET function is deleterious to plants, we developed an inducible expression system to study AtNEET function in mature plants using a time-course proteomics approach. Here, we report that the suppression of AtNEET cluster transfer function results in drastic changes in the expression of different members of the ferredoxin (Fd), Fd-thioredoxin (TRX) reductase (FTR), and TRX network of Arabidopsis, as well as in cytosolic cluster assembly proteins. In addition, the expression of Yellow Stripe-Like 6 (YSL6), involved in iron export from chloroplasts was elevated. Taken together, our findings reveal new roles for AtNEET in supporting the Fd-TFR-TRX network of plants, iron mobilization from the chloroplast, and cytosolic 2Fe-2S cluster assembly. In addition, we show that the AtNEET function is linked to the expression of glutathione peroxidases (GPXs), which play a key role in the regulation of ferroptosis and redox balance in different organisms. | ca_CA |
dc.format.extent | 21 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | MDPI | ca_CA |
dc.relation.isPartOf | Antioxidants, 2022, vol. 11, no 8 | ca_CA |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | ca_CA |
dc.subject | arabidopsis | ca_CA |
dc.subject | chloroplast | ca_CA |
dc.subject | inducible expression | ca_CA |
dc.subject | iron–sulfur | ca_CA |
dc.subject | NEET | ca_CA |
dc.subject | proteomics | ca_CA |
dc.subject | ROS | ca_CA |
dc.subject | thioredoxin | ca_CA |
dc.title | The Cluster Transfer Function of AtNEET Supports the Ferredoxin–Thioredoxin Network of Plant Cells | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.3390/antiox11081533 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://www.mdpi.com/2076-3921/11/8/1533 | ca_CA |
dc.description.sponsorship | This work was supported by funding from the National Science Foundation (IOS-2110017, IOS-1353886, MCB-1936590, IOS-1932639, MCB-2224839), the Bond Life Sciences Early Concept Grant, and the Interdisciplinary Plant Group, and the University of Missouri. | |
dc.description.sponsorship | Proteomic analyses were performed by The Charles W Gehrke Proteomics Center at the University of Missouri, Columbia, Missouri, USA (http://proteomics.missouri.edu, accessed on 5 April 2022). | |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
project.funder.name | National Science Foundation | ca_CA |
oaire.awardNumber | IOS-2110017 | ca_CA |
oaire.awardNumber | IOS-1353886 | ca_CA |
oaire.awardNumber | MCB-1936590 | ca_CA |
oaire.awardNumber | IOS-1932639 | ca_CA |
oaire.awardNumber | MCB-2224839 | ca_CA |
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