Oxidative-Induced Angiogenesis Is Modulated by Small Extracellular Vesicle miR-302a-3p Cargo in Retinal Pigment Epithelium Cells
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Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/36080
comunitat-uji-handle3:10234/36082
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Title
Oxidative-Induced Angiogenesis Is Modulated by Small Extracellular Vesicle miR-302a-3p Cargo in Retinal Pigment Epithelium CellsAuthor (s)
Date
2022-05Publisher
MDPIISSN
2076-3921Bibliographic citation
Oltra, M.; Martínez-Santos, M.; Ybarra, M.; Rowland, H.; Muriach, M.; Romero, J.; Sancho-Pelluz, J.; Barcia, J.M. Oxidative-Induced Angiogenesis Is Modulated by Small Extracellular Vesicle miR-302a-3p Cargo in Retinal Pigment Epithelium Cells. Antioxidants 2022, 11, 818. https://doi.org/10.3390/antiox11050818Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/2076-3921/11/5/818/htmVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Extracellular vesicles are released from cells under diverse conditions. Widely studied in cancer, they are associated with different diseases playing major roles. Recent reports indicate that oxidative damage promotes ... [+]
Extracellular vesicles are released from cells under diverse conditions. Widely studied in cancer, they are associated with different diseases playing major roles. Recent reports indicate that oxidative damage promotes the release of small extracellular vesicle (sEVs) from the retinal pigment epithelium (RPE), with an angiogenic outcome and changes in micro-RNA (miRNA) levels. The aim of this study was to determine the role of the miRNA miR-302a-3p, included within RPE-released sEVs, as an angiogenic regulator in cultures of endothelial cells (HUVEC). ARPE-19 cell cultures, treated with H2O2 to cause an oxidative insult, were transfected with a miR-302a-3p mimic. Later, sEVs from the medium were isolated and added into HUVEC or ARPE-19 cultures. sEVs from ARPE-19 cells under oxidative damage presented a decrease of miR-302a-3p levels and exhibited proangiogenic properties. In contrast, sEVs from miR-302a-3p-mimic transfected cells resulted in control angiogenic levels. The results herein indicate that miR-302a-3p contained in sEVs can modify VEGFA mRNA expression levels as part of its antiangiogenic features. [-]
Is part of
Antioxidants, 2022, vol. 11, no 5Funder Name
Ministerio de Ciencia, Innovación y Universidades | Instituto de Salud Carlos III
Funder ID
http://dx.doi.org/10.13039/501100011033
Project code
MICIU/ICTI2017-2020/MPID2020-117875GB-I00 | PI21/00083
Project title or grant
miRNAs como moduladores de la neovascularización en la retinopatía diabética
Rights
info:eu-repo/semantics/openAccess
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