Oxidative-Induced Angiogenesis Is Modulated by Small Extracellular Vesicle miR-302a-3p Cargo in Retinal Pigment Epithelium Cells
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INVESTIGACIONMetadades
Títol
Oxidative-Induced Angiogenesis Is Modulated by Small Extracellular Vesicle miR-302a-3p Cargo in Retinal Pigment Epithelium CellsAutoria
Data de publicació
2022-05Editor
MDPIISSN
2076-3921Cita bibliogràfica
Oltra, M.; Martínez-Santos, M.; Ybarra, M.; Rowland, H.; Muriach, M.; Romero, J.; Sancho-Pelluz, J.; Barcia, J.M. Oxidative-Induced Angiogenesis Is Modulated by Small Extracellular Vesicle miR-302a-3p Cargo in Retinal Pigment Epithelium Cells. Antioxidants 2022, 11, 818. https://doi.org/10.3390/antiox11050818Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://www.mdpi.com/2076-3921/11/5/818/htmVersió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Extracellular vesicles are released from cells under diverse conditions. Widely studied in cancer, they are associated with different diseases playing major roles. Recent reports indicate that oxidative damage promotes ... [+]
Extracellular vesicles are released from cells under diverse conditions. Widely studied in cancer, they are associated with different diseases playing major roles. Recent reports indicate that oxidative damage promotes the release of small extracellular vesicle (sEVs) from the retinal pigment epithelium (RPE), with an angiogenic outcome and changes in micro-RNA (miRNA) levels. The aim of this study was to determine the role of the miRNA miR-302a-3p, included within RPE-released sEVs, as an angiogenic regulator in cultures of endothelial cells (HUVEC). ARPE-19 cell cultures, treated with H2O2 to cause an oxidative insult, were transfected with a miR-302a-3p mimic. Later, sEVs from the medium were isolated and added into HUVEC or ARPE-19 cultures. sEVs from ARPE-19 cells under oxidative damage presented a decrease of miR-302a-3p levels and exhibited proangiogenic properties. In contrast, sEVs from miR-302a-3p-mimic transfected cells resulted in control angiogenic levels. The results herein indicate that miR-302a-3p contained in sEVs can modify VEGFA mRNA expression levels as part of its antiangiogenic features. [-]
Publicat a
Antioxidants, 2022, vol. 11, no 5Entitat finançadora
Ministerio de Ciencia, Innovación y Universidades | Instituto de Salud Carlos III
Identificador de l'entitat finançadora
http://dx.doi.org/10.13039/501100011033
Codi del projecte o subvenció
MICIU/ICTI2017-2020/MPID2020-117875GB-I00 | PI21/00083
Títol del projecte o subvenció
miRNAs como moduladores de la neovascularización en la retinopatía diabética
Drets d'accés
info:eu-repo/semantics/openAccess
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- MED_Articles [662]