Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic study
Impacte
Scholar |
Altres documents de l'autoria: Cerqueira, Andreia; García-Arnáez, Iñaki; Romero-Gavilán, Francisco J; Azkargorta, Mikel; Elortza, Felix; Martín de Llanos, José Javier; Carda, Carmen; GURRUCHAGA, MARILO; Goñi, Isabel; Suay, Julio
Metadades
Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7034
comunitat-uji-handle3:10234/8619
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INVESTIGACIONMetadades
Títol
Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic studyAutoria
Data de publicació
2022Editor
ElsevierISSN
2772-9508Cita bibliogràfica
A. Cerqueira, I. García-Arnáez, F. Romero-Gavilán, M. Azkargorta, F. Elortza, J. J. Martín de Llanos, C. Carda, M. Gurruchaga, I. Goñi, J. Suay, Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic study, Biomater. Adv., (2022), 212826, https://doi.org/10.1016/j.bioadv.2022.212826.Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://www.sciencedirect.com/science/article/pii/S2772950822001030Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
The cell–biomaterial interface is highly complex; thousands of molecules and many processes participate in its formation. Growing demand for improved biomaterials has highlighted the need to understand the structure ... [+]
The cell–biomaterial interface is highly complex; thousands of molecules and many processes participate in its formation. Growing demand for improved biomaterials has highlighted the need to understand the structure and functions of this interface. Proteomic methods offer a viable alternative to the traditional in vitro techniques for analyzing such systems. Magnesium is a promoter of cell adhesion and osteogenesis. Here, we used the LC-MS/MS to compare the protein expression profiles of human osteoblasts (HOb) exposed to sol-gel coatings without (MT) and with Mg (MT1.5Mg) for 1, 3, and 7 days. PANTHER, DAVID, and IPA databases were employed for protein identification and data analysis. Confocal microscopy and gene expression analysis were used for further characterization. Exposure to MT1.5Mg increased the HOb cell area and the expression of SP7, RUNX2, IBP3, COL3A1, MXRA8, and FBN1 genes. Proteomic analysis showed that MT1.5Mg affected the early osteoblast maturation (PI3/AKT, mTOR, ERK/MAPK), insulin metabolism, cell adhesion (integrin, FAK, actin cytoskeleton regulation) and oxidative stress pathways. Thus, the effects of Mg on cell adhesion and osteogenesis are rather complex, affecting several pathways rather than single processes. Our analysis also confirms the potential of proteomics in biomaterial characterization, showing a good correlation with in vitro results. [-]
Publicat a
Biomaterials Advances, 2022Entitat finançadora
Ministerio de Ciencia, Innovación y Universidades | Generalitat Valenciana | Universitat Jaume I | Basque Government
Codi del projecte o subvenció
MICIU/ICTI2017-2020/PID2020-113092RB-C21 | GRISOLIAP/2018/091 | APOSTD/2020/036 | PROMETEO/2020/069 | UJI-B2021-25 | PRE_2017_2_0044
Drets d'accés
info:eu-repo/semantics/openAccess
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