Viability, Stability and Biocontrol Activity in Planta of Specific Ralstonia solanacearum Bacteriophages after Their Conservation Prior to Commercialization and Use
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INVESTIGACIONMetadatos
Título
Viability, Stability and Biocontrol Activity in Planta of Specific Ralstonia solanacearum Bacteriophages after Their Conservation Prior to Commercialization and UseAutoría
Fecha de publicación
2022-01Editor
MDPIISSN
1999-4915Cita bibliográfica
Álvarez, B.; Gadea-Pallás, L.; Rodríguez, A.; Vicedo, B.; Figàs-Segura, À.; Biosca, E.G. Viability, Stability and Biocontrol Activity in Planta of Specific Ralstonia solanacearum Bacteriophages after Their Conservation Prior to Commercialization and Use. Viruses 2022, 14, 183. https://doi.org/ 10.3390/v14020183Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.mdpi.com/1999-4915/14/2/183Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Ralstonia solanacearum is a pathogen that causes bacterial wilt producing severe damage
in staple solanaceous crops. Traditional control has low efficacy and/or environmental impact.
Recently, the bases of a new ... [+]
Ralstonia solanacearum is a pathogen that causes bacterial wilt producing severe damage
in staple solanaceous crops. Traditional control has low efficacy and/or environmental impact.
Recently, the bases of a new biotechnological method by lytic bacteriophages vRsoP-WF2, vRsoPWM2 and vRsoP-WR2 with specific activity against R. solanacearum were established. However, some
aspects remain unknown, such as the survival and maintenance of the lytic activity after submission
to a preservation method as the lyophilization. To this end, viability and stability of lyophilized
vRsoP-WF2, vRsoP-WM2 and vRsoP-WR2 and their capacity for bacterial wilt biocontrol have been
determined against one pathogenic Spanish reference strain of R. solanacearum in susceptible tomato
plants in different conditions and making use of various cryoprotectants. The assays carried out have
shown satisfactory results with respect to the viability and stability of the bacteriophages after the
lyophilization process, maintaining high titers throughout the experimental period, and with respect
to the capacity of the bacteriophages for the biological control of bacterial wilt, controlling this disease
in more than 50% of the plants. The results offer good prospects for the use of lyophilization as a
conservation method for the lytic bacteriophages of R. solanacearum in view of their commercialization
as biocontrol agents. [-]
Publicado en
Viruses, vol. 14, núm. 2 (2022)Entidad financiadora
MCIN/AEI/10.13039/501100011033, the Spanish Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) | “ERDF A way of making Europe"
Código del proyecto o subvención
RTA2015-00087-C02
Derechos de acceso
info:eu-repo/semantics/openAccess
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