Engineered axon tracts within tubular biohybrid scaffolds
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Title
Engineered axon tracts within tubular biohybrid scaffoldsAuthor (s)
Date
2021-08-12Publisher
IOP PublishingISSN
1741-2560; 1741-2552Bibliographic citation
Doblado LR, Martínez-Ramos C, García-Verdugo JM, Moreno-Manzano V, Pradas MM. Engineered axon tracts within tubular biohybrid scaffolds. J Neural Eng. 2021 Aug 12;18(4). doi: 10.1088/1741-2552/ac17d8. PMID: 34311448.Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/submittedVersionSubject
Abstract
Injuries to the nervous system that involve the disruption of axonal pathways are devastating to the individual and require specific tissue engineering strategies. Here we analyse a cells-biomaterials strategy to ... [+]
Injuries to the nervous system that involve the disruption of axonal pathways are devastating to the individual and require specific tissue engineering strategies. Here we analyse a cells-biomaterials strategy to overcome the obstacles limiting axon regenerationin vivo, based on the combination of a hyaluronic acid (HA) single-channel tubular conduit filled with poly-L-lactide acid (PLA) fibres in its lumen, with pre-cultured Schwann cells (SCs) as cells supportive of axon extension. The HA conduit and PLA fibres sustain the proliferation of SC, which enhance axon growth acting as a feeder layer and growth factor pumps. The parallel unidirectional ensemble formed by PLA fibres and SC tries to recapitulate the directional features of axonal pathways in the nervous system. A dorsal root ganglion (DRG) explant is planted on one of the conduit's ends to follow axon outgrowth from the DRG. After a 21 d co-culture of the DRG + SC-seeded conduit ensemble, we analyse the axonal extension throughout the conduit by scanning, transmission electronic and confocal microscopy, in order to study the features of SC and the grown axons and their association. The separate effects of SC and PLA fibres on the axon growth are also experimentally addressed. The biohybrid thus produced may be considered a synthetic axonal pathway, and the results could be of use in strategies for the regeneration of axonal tracts. [-]
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J Neural Eng . 2021 Aug 12;18(4). doi: 10.1088/1741-2552/ac17d8Funder Name
Ministerio de Economía y Competitividad (Spain) | Generalitat Valenciana | Ministerio de Educación, Cultura y Deporte
Project code
RTI2018-095872-B-C21 and C22/ERDF | PROMETEO/2019/075 | TerCel-RD16/0011/0026 | FPU15/04975
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© 2021 IOP Publishing Ltd.
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