Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatography
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Otros documentos de la autoría: Goberna Bravo, Mª Angeles; Albiol Chiva, Jaume; Vives-Peris, Vicente; Carda-Broch, Samuel; Esteve-Romero, Josep
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https://doi.org/10.1016/j.microc.2021.106317 |
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Título
Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatographyAutoría
Fecha de publicación
2021-04-22Editor
ElsevierCita bibliográfica
GOBERNA-BRAVO, María Ángeles, et al. Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatography. Microchemical Journal, 2021, vol. 167, p. 106317.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
It is no doubt Isoniazid is a powerful tuberculosis drug, but it might give rise to Vitamin B6 (Pyridoxine) deficiency. In this case, a usual treatment is the combined administration of Isoniazid and Pyridoxine. An ... [+]
It is no doubt Isoniazid is a powerful tuberculosis drug, but it might give rise to Vitamin B6 (Pyridoxine) deficiency. In this case, a usual treatment is the combined administration of Isoniazid and Pyridoxine. An easy-to-conduct procedure based on Micellar Liquid Chromatography has been developed to quantify Isoniazid and Pyridoxine in plasma from Tuberculosis patients. The sample was diluted in mobile phase, filtered and directly injected, thus avoiding extraction or purification steps. Both drugs were adequately resolved from the matrix and endogenous compounds using a mobile phase made up of 0.15 M sodium dodecyl sulfate – 8%(v/v) 1-butanol – 0.01 M phosphate buffer at pH 3, running at 1 mL/min through a C18 column at 25 °C, in less than 8 min. Detection was carried out by UV-Absorbance at 265 nm. This innovative analytical method was validated following the Food and Drug Administration Bioanalytical Method Validation Guidance for Industry (2018) with satisfactory results. The response of the drugs was linear in the range 0.1 to 15 mg/L, with r2 > 0.9990 for both compounds. Trueness and precision were 89.2–102.3% and <8.5%, respectively. Limits of detection was 0.03 mg/L and no significant carry-over effect was noticed. Finally, the analytical method was used to determine both compounds in plasma samples of Tuberculosis patients and for stability studies. [-]
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Microchemical Journal Vol. 167, August 2021Entidad financiadora
Universitat Jaume I
Código del proyecto o subvención
UJI-B2-018-20
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© 2021 Elsevier B.V. All rights reserved.
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