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Enzymatic Δ1-Dehydrogenation of 3-Ketosteroids—Reconciliation of Kinetic Isotope Effects with the Reaction Mechanism
dc.contributor.author | Glanowski, Michał | |
dc.contributor.author | Wójcik, Patrycja | |
dc.contributor.author | Procner, Magdalena | |
dc.contributor.author | Borowski, Tomasz | |
dc.contributor.author | Lupa, Dawid | |
dc.contributor.author | Mielczarek, Przemysław | |
dc.contributor.author | Oszajca, Maria | |
dc.contributor.author | Świderek, Katarzyna | |
dc.contributor.author | Moliner, Vicent | |
dc.contributor.author | Bojarski, Andrzej | |
dc.contributor.author | Szaleniec, Maciej | |
dc.date.accessioned | 2021-11-03T09:06:29Z | |
dc.date.available | 2021-11-03T09:06:29Z | |
dc.date.issued | 2021-07-02 | |
dc.identifier.citation | Glanowski, M., Wójcik, P., Procner, M., Borowski, T., Lupa, D., Mielczarek, P., Oszajca, M., Świderek, K.; Moliner, V.; Bojarski, A. J.; Szaleniec, M. Enzymatic Δ1-Dehydrogenation of 3-Ketosteroids—Reconciliation of Kinetic Isotope Effects with the Reaction Mechanism. ACS Catalysis. 2021, 11 (13), 8211-8225, DOI: 10.1021/acscatal.1c01479 | ca_CA |
dc.identifier.issn | 2155-5435 | |
dc.identifier.uri | http://hdl.handle.net/10234/195360 | |
dc.description.abstract | Δ1-Dehydrogenation of 3-ketosteroids catalyzed by flavin adenine dinucleotide (FAD)-dependent 3-ketosteroid dehydrogenases (Δ1-KSTD) is a crucial step in steroid degradation and synthesis of several steroid drugs. The catalytic mechanism assumes the formation of a double bond in two steps, proton abstraction by tyrosyl ion, and a rate-limiting hydride transfer to FAD. This hypothesis was never verified by quantum-mechanical studies despite contradictory results from the kinetic isotope effect (KIE) reported in 1960 by Jerussi and Ringold [Biochemistry1965, 4 (10)]. In this paper, we present results that reconcile the mechanistic hypothesis with experimental evidence. Quantum mechanics/molecular mechanics molecular dynamics simulations show that the proposed mechanism is indeed the most probable, but barriers associated with substrate activation (13.4–16.3 kcal·mol–1) and hydride transfer (15.5–18.0 kcal·mol–1) are very close (1.7–2.1 kcal·mol–1), which explains normal KIE values for steroids labeled either at C1 or C2 atoms. We confirm that tyrosyl ion acting as the catalytic base is indeed necessary for efficient activation of the steroid. We explain the lower value of the observed KIE (1.5–3.5) by the nature of the free energy surface, the presence of diffusion limitation, and to a smaller extent, conformational changes of the enzyme upon substrate binding. Finally, we confirm the Ping-Pong bi–bi kinetics of the whole Δ1-dehydrogenation and demonstrate that substrate binding, steroid dehydrogenation, and enzyme reoxidation proceed at comparable rates. | ca_CA |
dc.format.extent | 15 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | American Chemical Society | ca_CA |
dc.relation.isPartOf | ACS Catalysis, 2021, vol. 11, no 13 | ca_CA |
dc.rights.uri | http://creativecommons.org/licenses/by-sa/4.0/ | ca_CA |
dc.subject | Δ1-ketosteroid dehydrogenase | ca_CA |
dc.subject | 3-ketosteroids | ca_CA |
dc.subject | Δ1-dehydrogenation | ca_CA |
dc.subject | kinetic isotope effect | ca_CA |
dc.subject | QM/MM | ca_CA |
dc.subject | kinetic solvent viscosity effect | ca_CA |
dc.subject | Ping-Pong bi−bi mechanism | ca_CA |
dc.title | Enzymatic Δ1-Dehydrogenation of 3-Ketosteroids—Reconciliation of Kinetic Isotope Effects with the Reaction Mechanism | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.1021/acscatal.1c01479 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://pubs.acs.org/doi/abs/10.1021/acscatal.1c01479 | ca_CA |
dc.description.sponsorship | The authors acknowledge partial financial support from the National Science Centre Poland under the OPUS grant number UMO-2016/21/B/ST4/03798. M.G and P.W. acknowledge the fellowship with project no. POWR. 03.02.00-00-I013/16. This research was supported in part by PLGrid (CYFRONET) Infrastructure, by the Spanish Ministerio de Ciencia e Innovación (grant PGC2018-094852-B-C21 and PID2019-107098RJ-I00), the Generalitat Valenciana (grant AICO/2019/195 and SEJI/2020/007) and Universitat Jaume I (grant UJI·B2020-031 and UJI-A2019-04). We acknowledge the joint consortium “Interdisciplinary Centre of Physical, Chemical and Biological Sciences” of ICSC PAS and INP PAS for providing access to the Agilent 1290 Infinity System with an automatic autosampler and an M.S. Agilent 6460 Triple Quad Detector. | |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
project.funder.name | National Science Centre Poland under the OPUS | ca_CA |
project.funder.name | Ministerio de Ciencia e Innovación | ca_CA |
project.funder.name | Generalitat Valenciana | ca_CA |
project.funder.name | Universitat Jaume I | ca_CA |
oaire.awardNumber | UMO-2016/21/B/ST4/03798 | ca_CA |
oaire.awardNumber | POWR. 03.02.00-00-I013/16 | ca_CA |
oaire.awardNumber | PGC2018-094852-B-C21 | ca_CA |
oaire.awardNumber | PID2019-107098RJ-I00 | ca_CA |
oaire.awardNumber | AICO/2019/195 | ca_CA |
oaire.awardNumber | SEJI/2020/007 | ca_CA |
oaire.awardNumber | UJI·B2020-031 | ca_CA |
oaire.awardNumber | UJI-A2019-04 | ca_CA |
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