Multi-Physical Parameter Cross-Sectional Imaging of Quantitative Phase and Fluorescence by Integrated Multimodal Microscopy
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INVESTIGACIONMetadata
Title
Multi-Physical Parameter Cross-Sectional Imaging of Quantitative Phase and Fluorescence by Integrated Multimodal MicroscopyAuthor (s)
Date
2021-03-11Publisher
IEEEISSN
1077-260X; 1558-4542Bibliographic citation
S. K. Rajput et al., "Multi-Physical Parameter Cross-Sectional Imaging of Quantitative Phase and Fluorescence by Integrated Multimodal Microscopy," in IEEE Journal of Selected Topics in Quantum Electronics, vol. 27, no. 4, pp. 1-9, July-Aug. 2021, Art no. 6801809, doi: 10.1109/JSTQE.2021.3064406.Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Integrated multimodal cross-sectional or volumetric
imaging techniques give us fruitful information to understand the
behavior or status of target objects such as biological samples. Most
of the reported systems ... [+]
Integrated multimodal cross-sectional or volumetric
imaging techniques give us fruitful information to understand the
behavior or status of target objects such as biological samples. Most
of the reported systems for this purpose are either time consuming
due to scanning or use additional reference beams such as in
interferometry. Therefore, fast, simple, highly efficient, and powerful multimodal imaging systems that can perform cross-sectional
imaging with simple algorithms are worth to be investigated. In
this paper, a multimodal technique for cross-sectional quantitative
phase and fluorescence imaging with computational microscopy
is presented. We combine cross-sectional fluorescence and quantitative phase imaging by using the transport of intensity equation
(TIE) and numerical wave propagation. The amplitude and phase
of the fluorescence light wave with partially spatial coherence are
obtained from three defocused intensity patterns. The proposed
hybrid imaging system is simple, compact, and non-iterative. We
present experimental results of microbeads and fluorescent proteinlabeled living cells of the moss Physcomitrella patensto demonstrate
the performance of the proposed imaging system. [-]
Is part of
IEEE Journal of Selected Topics in Quantum Electronics, Vol. 27, Issue: 4 (July-Aug. 2021)Funder Name
Japan Society for the Promotion of Science | JST CREST | National Institutes of Natural Sciences | Generalitat Valenciana | Ministerio de Ciencia e Innovación (España)
Project code
10.13039/501100001691 | 10.13039/501100006321 | 10.13039/501100003359 | 10.13039/501100004837
Rights
info:eu-repo/semantics/openAccess
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