Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode
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Other documents of the author: moradi, Aboubakr; El-Shetehy, Mohamed; Gamir, Jordi; Austerlitz, Tina; Dahlin, Paul; Wieczorek, Krzysztof; Künzler, Markus; Mauch, Felix
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Title
Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic NematodeAuthor (s)
Date
2021-04-09Publisher
Frontiers MediaISSN
1664-462XBibliographic citation
Moradi A, El-Shetehy M, Gamir J, Austerlitz T, Dahlin P, Wieczorek K, Künzler M and Mauch F (2021) Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode. Front. Plant Sci. 12:657451. doi: 10.3389/fpls.2021.657451Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Coprinopsis cinerea lectin 2 (CCL2) is a fucoside-binding lectin from the basidiomycete
C. cinerea that is toxic to the bacterivorous nematode Caenorhabditis elegans as well
as animal-parasitic and fungivorous ... [+]
Coprinopsis cinerea lectin 2 (CCL2) is a fucoside-binding lectin from the basidiomycete
C. cinerea that is toxic to the bacterivorous nematode Caenorhabditis elegans as well
as animal-parasitic and fungivorous nematodes. We expressed CCL2 in Arabidopsis
to assess its protective potential toward plant-parasitic nematodes. Our results
demonstrate that expression of CCL2 enhances host resistance against the cyst
nematode Heterodera schachtii. Surprisingly, CCL2-expressing plants were also more
resistant to fungal pathogens including Botrytis cinerea, and the phytopathogenic
bacterium Pseudomonas syringae. In addition, CCL2 expression positively affected
plant growth indicating that CCL2 has the potential to improve two important
agricultural parameters namely biomass production and general disease resistance. The
mechanism of the CCL2-mediated enhancement of plant disease resistance depended
on fucoside-binding by CCL2 as transgenic plants expressing a mutant version of
CCL2 (Y92A), compromised in fucoside-binding, exhibited wild type (WT) disease
susceptibility. The protective effect of CCL2 did not seem to be direct as the lectin
showed no growth-inhibition toward B. cinerea in in vitro assays. We detected, however,
a significantly enhanced transcriptional induction of plant defense genes in CCL2- but
not CCL2-Y92A-expressing lines in response to infection with B. cinerea compared
to WT plants. This study demonstrates a potential of fungal defense lectins in plant
protection beyond their use as toxins. [-]
Is part of
Frontiers in Plant Science 12:657451Funder Name
Swiss National Science Foundation | Austrian Science Fund
Project code
grant nos 31003A_129696 | 31003A_173097 | FWF, grant no P 29620-B25
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