BDNF‐Gene Transfected Schwann Cell‐Assisted Axonal Extension and Sprouting on New PLA–PPy Microfiber Substrates
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INVESTIGACIONMetadades
Títol
BDNF‐Gene Transfected Schwann Cell‐Assisted Axonal Extension and Sprouting on New PLA–PPy Microfiber SubstratesAutoria
Data de publicació
2021-03-01Editor
WileyISSN
1616-5187; 1616-5195Cita bibliogràfica
GISBERT ROCA, Fernando, et al. BDNF‐Gene Transfected Schwann Cell‐Assisted Axonal Extension and Sprouting on New PLA–PPy Microfiber Substrates. Macromolecular Bioscience, 2021, p. 2000391.Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://onlinelibrary.wiley.com/journal/16165195Versió
info:eu-repo/semantics/acceptedVersionParaules clau / Matèries
Resum
The work here reported analyzes the effect of increased efficiency of brain‐derived neurotrophic factor (BDNF) production by electroporated Schwann cells (SCs) on the axonal extension in a coculture system on a ... [+]
The work here reported analyzes the effect of increased efficiency of brain‐derived neurotrophic factor (BDNF) production by electroporated Schwann cells (SCs) on the axonal extension in a coculture system on a biomaterial platform that can be of interest for the treatment of injuries of the nervous system, both central and peripheral. Rat SCs are electrotransfected with a plasmid coding for the BDNF protein in order to achieve an increased expression and release of this protein into the culture medium of the cells, performing the best balance between the level of transfection and the number of living cells. Gene‐transfected SCs show an about 100‐fold increase in the release of BDNF into the culture medium, compared to nonelectroporated SCs. Cocultivation of electroporated SCs with rat dorsal root ganglia (DRG) is performed on highly aligned substrates of polylactic acid (PLA) microfibers coated with the electroconductive polymer polypyrrol (PPy). The coculture of DRG with electrotransfected SCs increase both the axonal extension and the axonal sprouting from DRG neurons compared to the coculture of DRG with nonelectroporated SCs. Therefore, the use of PLA–PPy highly aligned microfiber substrates preseeded with electrotransfected SCs with an increased BDNF secretion is capable of both guiding and accelerating axonal growth. [-]
Publicat a
Macromol. Biosci. 2021, 2000391Dades relacionades
mabi202000391-sup-0001-SuppMat.pdfEntitat finançadora
Ministerio de Ciencia, Innovación y Universidades (Spain) | Agencia Estatal de Investigación
Codi del projecte o subvenció
FPU16/01833, EST18/00524 | DPI2015‐72863‐EXP | RTI2018‐095872‐B‐C22/ERDF
Drets d'accés
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