Cromatography hyphenated to high resolution mass spectrometry in untargeted metabolomics for investigation of food (bio) markers

Abstract

Currently, there is a growing demand by our society, authorities and science to increase the knowledge about the food consumed and its relationship with health and disease. Untargeted metabolomics approaches are emerging as powerful tools for exploring metabolic changes in the biological system under different conditions, and it is known their potential in food studies. In addition, chromatography hyphenated to high resolution mass spectrometry provides sensitivity and selectivity enough to cover a wide range of metabolites in complex samples. This work is a part of a comprehensive study whose aim is to provide an overview of the most widely adopted method of high resolution mass spectrometry-based techniques for the identification of food (bio)markers through the untargeted metabolomics workflow. Detailed information is provided about the new trends in each stage from updated literature from the last three years and other reviews with the objective of helping researchers to optimize a proper methodology. This fragment covers study design, sample preparation and data acquisition, including additional sections of future prospects and applications.