Dynamic Changes in Ultrastructure of the Primary Cilium in Migrating Neuroblasts in the Postnatal Brain
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INVESTIGACIONMetadatos
Título
Dynamic Changes in Ultrastructure of the Primary Cilium in Migrating Neuroblasts in the Postnatal BrainAutoría
Fecha de publicación
2019-12-11Editor
Society for NeuroscienceCita bibliográfica
MATSUMOTO, Mami, et al. Dynamic changes in ultrastructure of the primary cilium in migrating neuroblasts in the postnatal brain. Journal of Neuroscience, 2019, 39.50: 9967-9988.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.jneurosci.org/content/39/50/9967.abstractVersión
info:eu-repo/semantics/submittedVersionPalabras clave / Materias
Resumen
New neurons, referred to as neuroblasts, are continuously generated in the ventricular-subventricular zone of the brain throughout an animal's life. These neuroblasts are characterized by their unique potential for ... [+]
New neurons, referred to as neuroblasts, are continuously generated in the ventricular-subventricular zone of the brain throughout an animal's life. These neuroblasts are characterized by their unique potential for proliferation, formation of chain-like cell aggregates, and long-distance and high-speed migration through the rostral migratory stream (RMS) toward the olfactory bulb (OB), where they decelerate and differentiate into mature interneurons. The dynamic changes of ultrastructural features in postnatal-born neuroblasts during migration are not yet fully understood. Here we report the presence of a primary cilium, and its ultrastructural morphology and spatiotemporal dynamics, in migrating neuroblasts in the postnatal RMS and OB. The primary cilium was observed in migrating neuroblasts in the postnatal RMS and OB in male and female mice and zebrafish, and a male rhesus monkey. Inhibition of intraflagellar transport molecules in migrating neuroblasts impaired their ciliogenesis and rostral migration toward the OB. Serial section transmission electron microscopy revealed that each migrating neuroblast possesses either a pair of centrioles or a basal body with an immature or mature primary cilium. Using immunohistochemistry, live imaging, and serial block-face scanning electron microscopy, we demonstrate that the localization and orientation of the primary cilium are altered depending on the mitotic state, saltatory migration, and deceleration of neuroblasts. Together, our results highlight a close mutual relationship between spatiotemporal regulation of the primary cilium and efficient chain migration of neuroblasts in the postnatal brain. [-]
Proyecto de investigación
Japan Agency for Medical Research and Development (AMED) (JP19bm0704033 [to K.S.], JP19gm1210007 [to K.S.], JP19jm0210060 [to M.S., N.K., and K.S.]), Japan Society for the Promotion of Science (JSPS) KAKENHI (26250019, 15H01384 15H01217, 16H06280, 17H01392, 17H05512, 17H05750, 19H04757, 19H04785, 18KK0213 [to K.S.], 26830014, 18K14823 [to M.S.], 16J00373 [to M.M.]), JSPS Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers (S2704 [to M.S., V.H-P., N.K., J.M.G-V., and K.S.]), Bilateral Open Partnership Joint Research Projects (to K.S.), Grant-in-Aid for Research at Nagoya City University (to K.S.), Cooperative Study Programs of National Institute for Physiological Sciences [to K.S.], the Mitsubishi Foundation (to K.S.), the Canon Foundation (to K.S.), the Takeda Science Foundation (to K.S. and M.S.), the Spanish Ministry of Science, Innovation and Universities (PCI2018-093062 [to V.H-P., and J.M.G-V.]), and the Independent Research Fund Denmark (8020-00083B [to K.K.]). M.M. was supported by a JSPS Fellowship.Derechos de acceso
Copyright © 2020 by the Society for Neuroscience.
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
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