Why Are Some Enzymes Dimers? Flexibility and Catalysis in Thermotoga maritima Dihydrofolate Reductase
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Title
Why Are Some Enzymes Dimers? Flexibility and Catalysis in Thermotoga maritima Dihydrofolate ReductaseDate
2019-05-13Publisher
American Chemical SocietyISSN
2155-5435Bibliographic citation
Ruiz-Pernía, J. Javier, Tuñón, Iñaki, Moliner, Vicent and Allemann, Rudolf K. 2019. Why are some enzymes dimers? flexibility and catalysis in Thermotoga maritima dihydrofolate reductase. ACS Catalysis 9 , pp. 5902-5911. 10.1021/acscatal.9b01250Type
info:eu-repo/semantics/articlePublisher version
https://pubs.acs.org/doi/abs/10.1021/acscatal.9b01250Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Dihydrofolate reductase from Thermotoga maritima (TmDFHFR) is a dimeric thermophilic enzyme that catalyzes the hydride transfer from the cofactor NADPH to dihydrofolate less efficiently than other DHFR enzymes, such ... [+]
Dihydrofolate reductase from Thermotoga maritima (TmDFHFR) is a dimeric thermophilic enzyme that catalyzes the hydride transfer from the cofactor NADPH to dihydrofolate less efficiently than other DHFR enzymes, such as the mesophilic analogue Escherichia coli DHFR (EcDHFR). Using QM/MM potentials, we show that the reduced catalytic efficiency of TmDHFR is most likely due to differences in the amino acid sequence that stabilize the M20 loop in an open conformation, which prevents the formation of some interactions in the transition state and increases the number of water molecules in the active site. However, dimerization provides two advantages to the thermophilic enzyme: it protects its structure against denaturation by reducing thermal fluctuations and it provides a less negative activation entropy, toning down the increase of the activation free energy with temperature. Our molecular picture is confirmed by the analysis of the temperature dependence of enzyme kinetic isotope effects in different DHFR enzymes. [-]
Is part of
ACS Catalysis, 2019, 9, 7, 5902-5911Investigation project
Spanish Ministerio de Economia y Competitividad and FEDER funds: PGC2018-094852-B-C2, CTQ2015-66223-C2, CTQ201S-74523-JIN(AEI/FEDER); Universitat Jaume I: UJI.B2017-31; Generalitat Valenciana: AICO/2018/238; United States Department of Health & Human Services National Institutes of Health (NIH) - USA: NIH R01 GM065368;Biotechnology and Biological Sciences Research Council (BBSRC): BB/J005266/1 BB/L020394/1Rights
info:eu-repo/semantics/openAccess
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