Inactivation of UDP-Glucose Sterol Glucosyltransferases Enhances Arabidopsis Resistance to Botrytis cinerea
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Other documents of the author: Castillo Vazquez, Nidia; Pastor, Victoria; Chávez, Ángel; Arró Robert, Montserrat; Boronat, Albert; Flors, Victor; Ferrer, Albert; ALTABELLA, TERESA
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Title
Inactivation of UDP-Glucose Sterol Glucosyltransferases Enhances Arabidopsis Resistance to Botrytis cinereaAuthor (s)
Date
2019-09-27Publisher
Frontiers MediaBibliographic citation
CASTILLO VAZQUEZ, Nidia; PASTOR, Victoria; CHÁVEZ, Ángel; ARRÓ ROBERT, Montserrat; BORONAT, Albert; FLORS, Víctor; FERRER, Albert; ALTABELLA, Teresa (2019). Inactivation of UDP-Glucose Sterol Glucosyltransferases Enhances Arabidopsis Resistance to Botrytis cinerea. Frontiers in Plant Science, v. 10Type
info:eu-repo/semantics/articlePublisher version
https://www.frontiersin.org/articles/10.3389/fpls.2019.01162/fullVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Free and glycosylated sterols are both structural components of the plasma membrane
that regulate their biophysical properties and consequently different plasma membraneassociated processes such as plant adaptation ... [+]
Free and glycosylated sterols are both structural components of the plasma membrane
that regulate their biophysical properties and consequently different plasma membraneassociated processes such as plant adaptation to stress or signaling. Several reports
relate changes in glycosylated sterols levels with the plant response to abiotic stress,
but the information about the role of these compounds in the response to biotic stress is
scarce. In this work, we have studied the response to the necrotrophic fungus Botrytis
cinerea in an Arabidopsis mutant that is severely impaired in steryl glycosides biosynthesis
due to the inactivation of the two sterol glucosyltransferases (UGT80A2 and UGT80B1)
reported in this plant. This mutant exhibits enhanced resistance against B. cinerea when
compared to wild-type plants, which correlates with increased levels of jasmonic acid
(JA) and up-regulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the
JA signaling pathway. Upon B. cinerea infection, the ugt80A2;B1 double mutant also
accumulates higher levels of camalexin, the major Arabidopsis phytoalexin, than wildtype plants. Camalexin accumulation correlates with enhanced transcript levels of several
cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators
WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of
camalexin is coordinately regulated at the transcriptional level. After fungus infection, the
expression of genes involved in the indole glucosinolate biosynthesis is also up-regulated
at a higher degree in the ugt80A2;B1 mutant than in wild-type plants. Altogether, the
results of this study show that glycosylated sterols play an important role in the regulation
of Arabidopsis response to B. cinerea infection and suggest that this occurs through
signaling pathways involving the canonical stress-hormone JA and the tryptophanderived secondary metabolites camalexin and possibly also indole glucosinolates [-]
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Frontiers in Plant Science (2019), v. 10Investigation project
Juan de la Cierva project IJCI-2015-24527;Rights
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