Metagenomic assessment of the microbial community and methanogenic pathways in biosolids from a municipal wastewater treatment plant in Medellín, Colombia
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Other documents of the author: Bedoya, Katherine; Coltell, Oscar; Cabarcas, Felipe; Alzate, Juan F.
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https://doi.org/10.1016/j.scitotenv.2018.08.119 |
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Title
Metagenomic assessment of the microbial community and methanogenic pathways in biosolids from a municipal wastewater treatment plant in Medellín, ColombiaDate
2019Publisher
ElsevierISSN
0048-9697; 1879-1026Bibliographic citation
Bedoya, Katherine, et al. "Metagenomic assessment of the microbial community and methanogenic pathways in biosolids from a municipal wastewater treatment plant in Medellín, Colombia." Science of the total environment, 2019, vol. 648, p. 572-581Type
info:eu-repo/semantics/articlePublisher version
https://www.sciencedirect.com/science/article/pii/S0048969718330833Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Abundance and diversity of microbial communities in biosolids are variable and poorly studied in the tropics, and
it is known that rainfall is one of the events that could affect the phylogenetic and functional ... [+]
Abundance and diversity of microbial communities in biosolids are variable and poorly studied in the tropics, and
it is known that rainfall is one of the events that could affect the phylogenetic and functional microbial structure.
In the present study, using NGS technics, we studied the microbial diversity as well as the methanogenesis path-
way in one of the largest WWTP in Colombia. Besides, we sampled and analyzed biosolids from rainy season and
dry season.
Phylogenetic classi
fi
cation showed a predominance of bacteria in both samples and difference in the dominant
groups depending on the rainfall season. Whereas
Pseudomonas
was the dominant bacteria in the dry season,
Coprothermobacter
was in the rainy season. Archaea abundance was higher in the rainy season (11.5%) doubling
dry season proportion. The bioreactor biogas production and total solids content showed similar results between
rainy and dry season at the sampling dates. The most abundant Archaea related with methanogenesis was
Methanosaeta
, which is a methanogenic microorganism that exclusively uses acetate to produce methane. More-
over, annotation ofthe methanogenicpathway inthe metagenomeshowedabundancein genes encoding Acetyl-
CoA synthetases(ACSS), an enzyme that catalyzes acetate activation. Ourresults suggest that the microbial diver-
sity was stable among the two time points tested, rainy season and dry season; and, although there were changes
in the microbial abundance of dominant bacterial species, anaerobic digester performance is not affected. [-]
Is part of
Science of the total environment, 2019, vol. 648, p. 572-581Investigation project
This project was funded by the Colombian Administrative Depart- ment of Science, Technology and Innovation, COLCIENCIAS grant number 111556933304 (CTO.535-2013), and Vicerrectoría de Investigación, Universidad de Antioquia, grant number CPT-1401. Oscar Coltell work was supported by the Spanish Ministry of Health (Instituto de Salud Carlos III) and the Ministerio de Economía y Competitividad-Fondo Europeo de Desarrollo Regional (grants CIBER 06/03, SAF2016-80532-R, and PRX17/00500); the Fundació La Marató de TV3 (grant 538/U/2016); and the University Jaume I (grants P1- 1B2013-54 and COGRUP/2016/06).Rights
© 2018 Elsevier B.V. All rights reserved.
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info:eu-repo/semantics/restrictedAccess
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