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Accurate and easy method for systemin quantifcation and examining metabolic changes under diferent endogenous levels
dc.contributor.author | Pastor, Victoria | |
dc.contributor.author | Sánchez-Bel, Paloma | |
dc.contributor.author | Gamir, Jordi | |
dc.contributor.author | Pozo, Maria J. | |
dc.contributor.author | Flors, Victor | |
dc.date.accessioned | 2018-06-25T09:27:20Z | |
dc.date.available | 2018-06-25T09:27:20Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1746-4811 | |
dc.identifier.uri | http://hdl.handle.net/10234/175320 | |
dc.description.abstract | Background: Systemin has been extensively studied since it was discovered and is described as a peptidic hormone in tomato plants and other Solanaceae. Jasmonic acid and systemin are proposed to act through a positive feedback loop with jasmonic acid, playing synergistic roles in response to both wounding and insect attack. Despite its biological relevance, most studies regarding the function of systemin in defence have been studied via PROSYSTEMIN (PROSYS) gene expression, which encodes the propeptide prosystemin that is later cleaved to systemin (SYS). Interest‑ ingly, hardly any studies have been based on quantifcation of the peptide. Results: In this study, a simple and accurate method for systemin quantifcation was developed to understand its impact on plant metabolism. The basal levels of systemin were found to be extremely low. To study the role of endogenous systemin on plant metabolism, systemin was quantifed in a transgenic line overexpressing the PROSYS gene (PS+) and in a silenced antisense line (PS−). We evaluated the relevance of systemin in plant metabolism by analysing the metabolomic profles of both lines compared to wildtype plants through untargeted metabolomic profling. Compounds within the lignan biosynthesis and tyrosine metabolism pathways strongly accumulated in PS+compared to wild-type plants and to plants from the PS− line. The exogenous treatments with SYS enhanced accumulation of lignans, which confrms the role of SYS in cell wall reinforcement. Unexpectedly, PS+plants displayed wild-type levels of jasmonic acid (JA) but elevated accumulation of 12-oxo-phytodienoic acid (OPDA), suggesting that PS+should not be used as an over-accumulator of JA in experimental setups. Conclusions: A simple method, requiring notably little sample manipulation to quantify the peptide SYS, is described. Previous studies were based on genetic changes. In our study, SYS accumulated at extremely low levels in wild-type tomato leaves, showed slightly higher levels in the PROSYSTEMIN-overexpressing plants and was absent in the silenced lines. These small changes have a signifcant impact on plant metabolism. SA and OPDA, but not JA, were higher in the PROSYS-overexpressing plants. | ca_CA |
dc.format.extent | 14 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | BioMed Central | ca_CA |
dc.relation.isPartOf | Pastor et al. Plant Methods (2018) 14:33 | ca_CA |
dc.rights | © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | ca_CA |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-sa/4.0/ | * |
dc.title | Accurate and easy method for systemin quantifcation and examining metabolic changes under diferent endogenous levels | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.1186/s13007-018-0301-z | |
dc.relation.projectID | P1- 1B2015-33 ; AGL2015-64990-C2-2-R ; AICO/2016/029 | ca_CA |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://plantmethods.biomedcentral.com/articles/10.1186/s13007-018-0301-z | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
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provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
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publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.