Validation of a procedure to quantify oxolinic acid, danofloxacin, ciprofloxacin and enrofloxacin in selected meats by micellar liquid chromatography according to EU Commission Decision 2002/657/EC
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http://dx.doi.org/10.1002/elps.201700159 |
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Title
Validation of a procedure to quantify oxolinic acid, danofloxacin, ciprofloxacin and enrofloxacin in selected meats by micellar liquid chromatography according to EU Commission Decision 2002/657/ECDate
2017-08Publisher
WileyBibliographic citation
PERIS VICENTE, Juan; TAYEB CHERIF, K.; CARDA-BROCH, Samuel; ESTEVE-ROMERO, Josep. Validation of a procedure to quantify oxolinic acid, danofloxacin, ciprofloxacin and enrofloxacin in selected meats by micellar liquid chromatography according to EU Commission Decision 2002/657/EC. Electrophoresis (2017), v. 18, issue 16, p. 2011-2017Type
info:eu-repo/semantics/articlePublisher version
http://onlinelibrary.wiley.com/doi/10.1002/elps.201700159/abstractVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
The suitability of an analytical method to determine oxolinic acid, danofloxacin,
ciprofloxacin and enrofloxacin in edible tissues, based on micellar liquid chromatogra-
phy coupled with fluorescence detection, to be ... [+]
The suitability of an analytical method to determine oxolinic acid, danofloxacin,
ciprofloxacin and enrofloxacin in edible tissues, based on micellar liquid chromatogra-
phy coupled with fluorescence detection, to be applied in chicken, turkey, duck, lamb,
goat, rabbit and horse muscle, is described. The method was fully matrix-matched in-lab
revalidated, for each antimicrobial drug and meat, following the guidelines of the EU
Commission Decision 2002/657/EC. The permitted limits were the maximum residue
limits stated by the EU Commission Regulation 37/2010. The results obtained for the
studied validation parameters were in agreement with the guidelines: selectivity (the an-
tibiotics were resolved), linearity (r
2
⬎ 0.995), limit of detection (0.004–0.02 mg/kg), limits
of quantification (0.01–0.05 mg/kg), calibration range (up to 0.5 mg/kg), recovery (89.5–
105.0%), precision (⬍8.3%), decision limit, detection capability, ruggedness, stability and
application to incurred samples. The method was found to be able to provide reliable con-
centrations with low uncertainty within a large interval, including the maximum residue
limits, and then was useful to find out prohibited contaminated samples. The method did
not require to be adapted for these matrices, and then it maintained its interesting advan-
tages: short-time, eco-friendly, safe, inexpensive, easy-to-conduct, minimal manipulation
and useful for routine analysis. [-]
Is part of
Electrophoresis (2017), v. 38, issue 16Investigation project
Project P1.1B2012-36, granted by the Universitat Jaume IRights
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info:eu-repo/semantics/restrictedAccess
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