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Evaluation of uncertainty sources in the determination of testosterone in urine by calibration-based and isotope dilution quantification using ultra high performance liquid chromatography tandem mass spectrometry
dc.contributor.author | Pitarch-Motellón, Jorge | |
dc.contributor.author | Roig-Navarro, Antoni F. | |
dc.contributor.author | Sancho, Juan V | |
dc.contributor.author | Ibáñez, Maria | |
dc.contributor.author | Fabregat-Cabello, Neus | |
dc.contributor.author | Pozo, Óscar J. | |
dc.contributor.author | Ventura, Rosa | |
dc.contributor.author | García Alonso, J. Ignacio | |
dc.contributor.author | Rodríguez González, Pablo | |
dc.contributor.author | González Gago, Adriana | |
dc.contributor.author | Ereño Artabe, Amaia | |
dc.contributor.author | Van Eenoo, Peter | |
dc.contributor.author | Deventer, Koen | |
dc.contributor.author | Dehnes, Yvette | |
dc.contributor.author | Rzeppa, Sebastian | |
dc.date.accessioned | 2017-06-06T10:39:35Z | |
dc.date.available | 2017-06-06T10:39:35Z | |
dc.date.issued | 2017-06 | |
dc.identifier.uri | http://hdl.handle.net/10234/167880 | |
dc.description.abstract | Three quantification methodologies, namely calibration with internal standard (Cal-IS, non-weighted), weighted calibration with internal standard (wCal-IS) and isotope pattern deconvolution (IPD) have been used for the determination of testosterone in urine by LC-MS/MS. Uncertainty has been calculated and compared for the three methodologies through intra- and inter-laboratory reproducibility assays. IPD showed the best performance for the intra-laboratory reproducibility, with RSD and combined uncertainty values below 4% and 9% respectively. wCal-IS showed similar performance, while Cal-IS where not constant and clearly worse at the lowest concentration assayed (2 ng/mL) reaching RSD values up to 16%. The inter-laboratory assay indicated similar results although wCal-IS RSD (20%) was higher than IPD (10%) and Cal-IS get worse with RSD higher than 40% for the lowest concentration level. Uncertainty budgets calculated for the three procedures revealed that intercept and slope were the most important factors contributing to uncertainty for Cal-IS. The main factors for wCal-IS and IPD were the volumes of sample and/or standard measured. | ca_CA |
dc.description.sponsorShip | The authors acknowledge financial support from the Generalitat Valenciana (Research group of excellence Prometeo II 2014/023 and Collaborative Research on Environment and Food Safety ISIC/2012/016), as well as University Jaume I for project PB1-1B2013-55. Finally, the authors are grateful to the Serveis Centrals d'Instrumentació Científica (SCIC) of University Jaume I for using Acquity and TQD instruments. | ca_CA |
dc.format.extent | 18 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Journal of Chromatography A, June 2017 | ca_CA |
dc.rights | © 2017 Elsevier B.V. All rights reserved. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | testosterone | ca_CA |
dc.subject | urine | ca_CA |
dc.subject | mass spectrometry | ca_CA |
dc.subject | liquid chromatography | ca_CA |
dc.title | Evaluation of uncertainty sources in the determination of testosterone in urine by calibration-based and isotope dilution quantification using ultra high performance liquid chromatography tandem mass spectrometry | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.1016/j.chroma.2017.05.072 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | http://www.sciencedirect.com/science/article/pii/S0021967317308300 | ca_CA |
dc.type.version | info:eu-repo/semantics/submittedVersion |
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