Simultaneous determination of main phenolic acids and flavonoids in tomato by micellar electrokinetic capillary electrophoresis
Ver/ Abrir
Impacto
Scholar |
Otros documentos de la autoría: Martí, Raúl; Valcárcel, Mercedes; Herrero Martínez, José Manuel; Cebolla-Cornejo, Jaime; Roselló, Salvador
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
comunitat-uji-handle4:
INVESTIGACIONMetadatos
Título
Simultaneous determination of main phenolic acids and flavonoids in tomato by micellar electrokinetic capillary electrophoresisAutoría
Fecha de publicación
2017-04-15Editor
ElsevierCita bibliográfica
MARTÍ RENAU, Raúl; VALCÁRCEL GERMES, Mercedes; HERRERO MARTÍNEZ, José Manuel; CEBOLLA CORNEJO, Jaime; ROSELLÓ RIPOLLÉS, Salvador. Simultaneous determination of main phenolic acids and flavonoids in tomato by micellar electrokinetic capillary electrophoresis. Food Chemistry (2017), v. 221, pp. 439-446Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://www.sciencedirect.com/science/article/pii/S0308814616317836Palabras clave / Materias
Resumen
A methodology for the determination of tomato phenolic acids and flavonoids has been developed combining MEKC and DAD detection. The influence on polyphenol separation of pH and background electrolyte, BGE (borax, ... [+]
A methodology for the determination of tomato phenolic acids and flavonoids has been developed combining MEKC and DAD detection. The influence on polyphenol separation of pH and background electrolyte, BGE (borax, acetonitrile, methanol and SDS concentrations), was studied and optimized using response surface methodology and weighted desirability function. Separation of polyphenols was achieved within 20 min at 15 °C using 11.3 mM borax and 11.2 mM SDS adjusted to pH 8.5 as BGE. Validation was performed using standards and tomato extracts. Recoveries ranged from 77 to 106%. Acceptable repeatabilities were obtained for peak area (%RSD <3.1% and <3.7%) and migration times (%RSD <0.2% and <1.4%) for intra- and inter-day respectively. Detection limits ranged between 0.8 and 3.8 mg kg−1. Five and seven of these polyphenols were determined in samples of tomato and related species. This methodology will be valuable tool in breeding programs, analyzing a large number of samples. [-]
Publicado en
Food Chemistry (2017), v. 221Derechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- CAMN_Articles [568]