Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO Treatment
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Other documents of the author: Fernández Crespo, Emma; González-Hernández, Ana I.; Scalschi, Loredana; Llorens, Eugenio; García Agustín, Pilar; Camañes, Gemma
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comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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INVESTIGACIONMetadata
Title
Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO TreatmentAuthor (s)
Date
2016Publisher
Bio-protocol LLCISSN
2331-8325Bibliographic citation
Fernández-Crespo, E., González-Hernández, A. I., Scalschi, L., Llorens, E., García-Agustín, P. and Camañes, G. (2016). Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO Treatment. Bio-protocol 6(21): e1987. DOI: 10.21769/BioProtoc.1987Type
info:eu-repo/semantics/articlePublisher version
http://www.bio-protocol.org/e1987Abstract
This protocol can be used to inhibit the biosynthesis of polyamines, specifically putrescine,
in tomato plants grown with NH4
+ as a solely N source. In general, polyamines are positively charged
small metabolites ... [+]
This protocol can be used to inhibit the biosynthesis of polyamines, specifically putrescine,
in tomato plants grown with NH4
+ as a solely N source. In general, polyamines are positively charged
small metabolites implicated in physiological processes, including organogenesis, embryogenesis, floral
initiation and development, leaf senescence, pollen tube growth, fruit development and ripening and
participate in the response to abiotic and biotic stresses (Tiburcio et al., 2014). Polyamines are
synthesized from amino acids by decarboxylation of ornithine or arginine by ornithine decarboxylase
(ODC) or arginine decarboxylase (ADC), respectively (Walters, 2003). Tomato plants grown with NH4
+
as the sole N source presented an increase of putrescine content in leaves (Fernández-Crespo et al.,
2015). To assess the importance of putrescine accumulation, DL-α-(Difluoromethyl)arginine (DFMA) and
DL-α-(Difluoromethyl)ornithine (DFMO), inhibitors of putrescine synthesis, were used as irreversible
inhibitors of ADC and ODC enzymes, respectively (Fallon and Phillips, 1988), with the purpose of
reducing cellular putrescine accumulation induced by NH4
+ nutrition.
The inhibitor solution containing 2 mM DFMA and 5 mM DFMO was applied directly to each pot during
the week prior to sample collection. Putrescine content was reduced by 35.3% in tomato plants grown
with NH4
+. [-]
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Bio-protocol, Vol 6, Iss 21, Nov 05, 2016Rights
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info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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