Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO Treatment
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Altres documents de l'autoria: Fernández Crespo, Emma; González-Hernández, Ana I.; Scalschi, Loredana; Llorens, Eugenio; García Agustín, Pilar; Camañes, Gemma
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Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO TreatmentAutoria
Data de publicació
2016Editor
Bio-protocol LLCISSN
2331-8325Cita bibliogràfica
Fernández-Crespo, E., González-Hernández, A. I., Scalschi, L., Llorens, E., García-Agustín, P. and Camañes, G. (2016). Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO Treatment. Bio-protocol 6(21): e1987. DOI: 10.21769/BioProtoc.1987Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://www.bio-protocol.org/e1987Resum
This protocol can be used to inhibit the biosynthesis of polyamines, specifically putrescine,
in tomato plants grown with NH4
+ as a solely N source. In general, polyamines are positively charged
small metabolites ... [+]
This protocol can be used to inhibit the biosynthesis of polyamines, specifically putrescine,
in tomato plants grown with NH4
+ as a solely N source. In general, polyamines are positively charged
small metabolites implicated in physiological processes, including organogenesis, embryogenesis, floral
initiation and development, leaf senescence, pollen tube growth, fruit development and ripening and
participate in the response to abiotic and biotic stresses (Tiburcio et al., 2014). Polyamines are
synthesized from amino acids by decarboxylation of ornithine or arginine by ornithine decarboxylase
(ODC) or arginine decarboxylase (ADC), respectively (Walters, 2003). Tomato plants grown with NH4
+
as the sole N source presented an increase of putrescine content in leaves (Fernández-Crespo et al.,
2015). To assess the importance of putrescine accumulation, DL-α-(Difluoromethyl)arginine (DFMA) and
DL-α-(Difluoromethyl)ornithine (DFMO), inhibitors of putrescine synthesis, were used as irreversible
inhibitors of ADC and ODC enzymes, respectively (Fallon and Phillips, 1988), with the purpose of
reducing cellular putrescine accumulation induced by NH4
+ nutrition.
The inhibitor solution containing 2 mM DFMA and 5 mM DFMO was applied directly to each pot during
the week prior to sample collection. Putrescine content was reduced by 35.3% in tomato plants grown
with NH4
+. [-]
Publicat a
Bio-protocol, Vol 6, Iss 21, Nov 05, 2016Drets d'accés
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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