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Pseudopeptidic fluorescent on-off pH sensor based on pyrene excimer emission: Imaging of acidic cellular organelles
dc.contributor.author | Faggi, Enrico | |
dc.contributor.author | Serra-Vinardell, Jenny | |
dc.contributor.author | Pandey, Mrituanjay D. | |
dc.contributor.author | Casas, Josefina | |
dc.contributor.author | Fabriàs, Gemma | |
dc.contributor.author | Luis, Santiago V. | |
dc.contributor.author | Alfonso Rodríguez, Ignacio | |
dc.date.accessioned | 2016-09-23T07:13:30Z | |
dc.date.available | 2016-09-23T07:13:30Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Faggi, E., Serra-Vinardell, J., Pandey, M. D., Casas, J., Fabriàs, G., Luis, S. V., & Alfonso, I. (2016). Pseudopeptidic fluorescent on-off pH sensor based on pyrene excimer emission: Imaging of acidic cellular organelles. Sensors and Actuators B: Chemical, 234, 633-640. | ca_CA |
dc.identifier.issn | 0925-4005 | |
dc.identifier.uri | http://hdl.handle.net/10234/162867 | |
dc.description.abstract | A pseudopeptidic compound comprising a central C2 symmetric bis(aminoamide) moiety derived from the amino acid serine and two pyrene groups at the ends, Et(SerPyr)2, has been efficiently prepared. In aqueous solution, it displays fluorescence bands typical of both monomer and excimer pyrene emission. In particular, the emission at 490 nm, due to the excimer, is strongly pH-dependent, with a linear 4.7-fold increase from pH 6.0 to pH 4.2. This feature makes Et(SerPyr)2 suitable for intracellular pH sensing in the acidic range. A fluorescence microscopy study revealed that Et(SerPyr)2 allows the visualization of acidic organelles in human lung adenocarcinoma A549 cells, as well as their pH changes over time. Other advantages of this probe include its easy and modular synthesis, good photostability, high quantum yield, fast optical response, large Stokes shift (ca. 145 nm) and low cytotoxicity. | ca_CA |
dc.description.sponsorShip | We acknowledge financial support from the Ministry of Economy and Competitiveness, Spain (CTQ2012-38543-C03, CTQ2014-54743-R, CTQ2015-68429-R and CTQ2015-70117-R projects), Generalitat de Catalunya (AGAUR, 2014 SGR 231), Generalitat Valenciana (PROMETEO/2012/020) and UJI (PI-1B2013-38). We thank Mr. Pedro Rayo for excellent technical assistance. | ca_CA |
dc.format.extent | 8 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Sensors and Actuators B: Chemical, 234 | ca_CA |
dc.rights | Copyright © Elsevier B.V. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | fluorescence | ca_CA |
dc.subject | pseudopeptides | ca_CA |
dc.subject | live cells | ca_CA |
dc.subject | pH sensor | ca_CA |
dc.subject | acidic organelles | ca_CA |
dc.subject | pyrene | ca_CA |
dc.title | Pseudopeptidic fluorescent on-off pH sensor based on pyrene excimer emission: Imaging of acidic cellular organelles | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1016/j.snb.2016.05.037 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://www.sciencedirect.com/science/article/pii/S0925400516307134 | ca_CA |
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