Stability of drug mixtures in postoperative analgesia. Estimation of their stability and plasma levels by LC-MS/MS : identification of transformation products and metabolites by LC-HRMS and LC-DAD
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/158176
comunitat-uji-handle2:10234/71345
comunitat-uji-handle3:10234/73147
comunitat-uji-handle4:
TFG-TFMEste recurso está restringido
Metadatos
Título
Stability of drug mixtures in postoperative analgesia. Estimation of their stability and plasma levels by LC-MS/MS : identification of transformation products and metabolites by LC-HRMS and LC-DADAutoría
Tutor/Supervisor
Sancho Llopis, Juan VicenteFecha de publicación
2016-06-28Editor
Universitat Jaume IResumen
The aim of this work was to determine if the multimodal analgesia by intravenous
infusion of the mixture of drugs composed by dipyrone, tramadol and metoclopramide is
an effective way of analgesia administration to ... [+]
The aim of this work was to determine if the multimodal analgesia by intravenous
infusion of the mixture of drugs composed by dipyrone, tramadol and metoclopramide is
an effective way of analgesia administration to postoperative patients.
Depending on the pain level suffered by the patient after a surgical intervention,
the department of Anaesthesiology, Resuscitation and Pain therapy of the General
University Hospital of Castellon must evaluate two procedures of drugs administration in
post-operatory analgesia. The first one is selected for patients with acute pain, and consist
on a mixture of 6 g of dipyrone, 300 mg of tramadol and 30 mg of metoclopramide in 500
mL of physiological saline and infused intravenously during 24 hours. The other one, 2 g
of dipyrone, 100 mg of tramadol and 10 mg of metoclopramide is dissolved in 100 mL of
saline and infused for 20 minutes every 8 hours to patients with moderate pain (the
template for the analgesic treatment order can be found in Figure S 1 of supplementary
information).
This work was divided in three sections:
The first step was to develop a Liquid Chromatography coupled to tandem
mass spectrometry (LC-MS/MS) method for simultaneous determination of dipyrone,
tramadol and metoclopramide in physiological saline with the purpose of performing a
stability study during 24 hours of the mixture prior to intravenous administration. In this
stability study, High Performance Liquid Chromatography coupled to triple quadrupole
mass spectrometry (HPLC-QqQ) was used, butsupplementary information about possible
transformation products was obtained from liquid chromatography coupled to high
resolution mass spectrometry (LC-HRMS) and HPLC-diode-array detector (HPLCDAD).
The next step was to develop and validate an analytical method by
HPLC-QqQ for quantification of tramadol, metoclopramide, dipyrone and their
metabolites in blood plasma of patients to make a pharmacokinetic study and compare
the therapeutic effect of the two procedures of analgesia administrated to post-operatory
patients.
The last study goal is to find out non-reported metoclopramide metabolites
to better understanding the human metabolism of that drug, since it was founded less
information about metabolism of metoclopramide compared to dipyrone or tramadol. To this end, blood plasma samples prior and after post-operatory analgesia administration
where analysed by LC-HRMS and compared. [-]
Palabras clave / Materias
Màster Universitari en Tècniques Cromatogràfiques Aplicades | Máster Universitario en Técnicas Cromatográficas Aplicadas | University Master's Degree in Applied Chromatographic Techniques | Analgésicos | Cromatografía líquida | Analgesics | Liquid chromatography | Analgèsics | Cromatografia de líquids
Descripción
Treball Final de Màster Universitari en Tècniques Cromatogràfiques Aplicades (Pla de 2013). Codi: SIY009. Curs: 2015/2016
Tipo de documento
info:eu-repo/semantics/masterThesisDerechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess