Rapid and reproducible determination of active gibberellins in citrus tissues by UPLC/ESI-MS/MS
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Other documents of the author: Manzi Fraga, Matías Jesús; Gomez-Cadenas, Aurelio; Arbona, Vicent
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INVESTIGACIONMetadata
Title
Rapid and reproducible determination of active gibberellins in citrus tissues by UPLC/ESI-MS/MSDate
2015xmlui.dri2xhtml.METS-1.0.item-edition
PostprintPublisher
ElsevierISSN
0981-9428; 1873-2690Bibliographic citation
Matías Manzi, Aurelio Gómez-Cadenas, Vicent Arbona, Rapid and reproducible determination of active gibberellins in citrus tissues by UPLC/ESI-MS/MS, Plant Physiology and Biochemistry, Volume 94, 2015, Pages 1-9, ISSN 0981-9428, https://doi.org/10.1016/j.plaphy.2015.04.015.Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S0981942815300164Subject
Abstract
Phytohormone determination is crucial to explain the physiological mechanisms during growth and
development. Therefore, rapid and precise methods are needed to achieve reproducible determination of
phytohormones. ... [+]
Phytohormone determination is crucial to explain the physiological mechanisms during growth and
development. Therefore, rapid and precise methods are needed to achieve reproducible determination of
phytohormones. Among many others, gibberellins (GAs) constitute a family of complex analytes as most
of them share similar structure and chemical properties although only a few hold biological activity
(namely GA1; GA3; GA4 and GA7). A method has been developed to extract GAs from plant tissues by
mechanical disruption using ultrapure water as solvent and, in this way, ion suppression was reduced
whereas sensitivity increased. Using this methodology, the four active GAs were separated and quantified
by UPLC coupled to MS/MS using the isotope-labeled internal standards [2
H2]-GA1 and [2
H2]-GA4. To sum
up, the new method provides a fast and reproducible protocol to determine bioactive GAs at low concentrations,
using minimal amounts of sample and reducing the use of organic solvents. [-]
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Plant Physiology and Biochemistry Volume 94, September 2015, Pages 1–9Rights
© 2015 Elsevier Masson SAS. All rights reserved.
info:eu-repo/semantics/openAccess
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