Nitric oxide sensitive fluorescent polymeric hydrogels showing negligible interference by dehydroascorbic acid
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http://dx.doi.org/10.1016/j.eurpolymj.2014.03.027 |
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Títol
Nitric oxide sensitive fluorescent polymeric hydrogels showing negligible interference by dehydroascorbic acidAutoria
Data de publicació
2014Editor
ElsevierISSN
0014-3057Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://www.sciencedirect.com/science/article/pii/S0014305714001074Paraules clau / Matèries
Resum
A series of nitric oxide (NO) sensitive polymeric hydrogels have been synthesized and their properties described. The new materials are based on a poly(2-hydroxyethyl methacrylate) matrix entrapping the DAF-FM (4-am ... [+]
A series of nitric oxide (NO) sensitive polymeric hydrogels have been synthesized and their properties described. The new materials are based on a poly(2-hydroxyethyl methacrylate) matrix entrapping the DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein) fluorescent probe. Some of the developed materials show sensitivities in the range ca. 50–75 nM towards NO dissolved in aerated phosphate buffer (PBS), being nitrous anhydride the reactive species under such conditions. Remarkably, the sensitivity of the fluorescent polymers is not influenced by dehydroascorbic acid, a molecule of biological importance, which has been reported to be a common interfering species capable to react with well known probes for NO. [-]
Publicat a
European Polymer Journal, 2014, vol. 55Drets d'accés
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
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