A photoinduced electron transfer-based nanoprobe as a marker of acidic organelles in mammalian cells
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Altres documents de l'autoria: Marín, María J.; Galindo, Francisco; Thomas, Paul; Wileman, Tom; Russell, David A.
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http://dx.doi.org/10.1007/s00216-013-6905-2 |
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Títol
A photoinduced electron transfer-based nanoprobe as a marker of acidic organelles in mammalian cellsData de publicació
2013-07Editor
SpringerCita bibliogràfica
MARÍN, M. J.; GALINDO HONRUBIA, F.; THOMAS, P.; WILEMAN, T.; RUSSELL, D. A. A photoinduced electron transfer-based nanoprobe as a marker of acidic organelles in mammalian cells. Analytical and Bioanalytical Chemistry, v. 405, issue 19 (July 2013), p. 6197-6207Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://link.springer.com/article/10.1007%2Fs00216-013-6905-2Paraules clau / Matèries
Resum
Photoinduced electron transfer (PET)-based molecular probes have been successfully used for the intracelular imaging of the pH of acidic organelles. In this study, we describe the synthesis and characterization of a ... [+]
Photoinduced electron transfer (PET)-based molecular probes have been successfully used for the intracelular imaging of the pH of acidic organelles. In this study, we describe the synthesis and characterization of a novel PET-based pH nanoprobe and its biological application for the signaling of acidic organelles in mammalian cells. A fluorescent ligand sensitive to pH via the PET mechanism that incorporates a thiolated moiety was synthesized and used to stabilize gold nanoparticles (2.4±0.6 nm), yielding a PET-based nanoprobe. The PET nanoprobe was unambiguously characterized by transmission electron microscopy, proton nuclear magnetic resonance, Fourier transform infrared,
ultraviolet-visible absorption, and steady-state/time-resolved fluorescence spectroscopies which confirmed the functionalization of the gold nanoparticles with the PETbased
ligand. Following a classic PET behavior, the fluorescence emission of the PET-based nanoprobe was quenched in alkaline conditions and enhanced in an acidic environment.
The PET-based nanoprobe was used for the intracellular imaging of acidic environments within Chinese hamster ovary cells by confocal laser scanning microscopy. The internalization of the nanoparticles by the cells was confirmed by confocal fluorescence images and also by recording the fluorescence emission spectra of the intracellular PETbased nanoprobe from within the cells. Co-localization experiments using a marker of acidic organelles, LysoTracker Red DND-99, and a marker of autophagosomes, GFP-LC3, confirmthat the PET-based nanoprobe acts as marker of acidic organelles and autophagosomes within mammalian cells. [-]
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Analytical and Bioanalytical Chemistry, v. 405, issue 19 (July 2013)Drets d'accés
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
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