Using exomarkers to assess mitochondrial reactive species in vivo
Impacto
Scholar |
Otros documentos de la autoría: Logan, Angela; Cochemé, Helena M.; Boon Li Pun, Pamela; Apostolova, Nadezda; Smith, Robin A. J.; Larsen, Lesley; Larsen, David S.; James, Andrew M.; Fearnley, Ian M.; Rogatti, Sebastian; Prime, Tracy A.; Finichiu, Peter G.; Dare, Anna; Chouchani, Edward T.; Pell, Victoria R.; Methner, Carmen; Quin, Caroline; McQuaker, Stephen J.; Krieg, Thomas; Hartley, Richard C.; Murphy, Michael P.
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http://dx.doi.org/10.1016/j.bbagen.2013.05.026 |
Metadatos
Título
Using exomarkers to assess mitochondrial reactive species in vivoAutoría
Fecha de publicación
2014-02Editor
ElsevierISSN
0304-4165Cita bibliográfica
LOGAN, Angela, et al. Using exomarkers to assess mitochondrial reactive species< i> in vivo</i>. Biochimica et Biophysica Acta (BBA)-General Subjects, 2014, vol. 1840, no 2, p. 923-930Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://www.sciencedirect.com/science/article/pii/S0304416513002225#Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Background
The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of ... [+]
Background
The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging.
Scope of review
One approach towards achieving this goal is the use of exomarkers. In this, exogenous probe compounds are administered to the intact organism and are then transformed by the reactive molecules in vivo to produce a diagnostic exomarker. The exomarker and the precursor probe can be analysed ex vivo to infer the identity and amounts of the reactive species present in vivo. This is akin to the measurement of biomarkers produced by the interaction of reactive species with endogenous biomolecules.
Major conclusions and general significance
Our laboratories have developed mitochondria-targeted probes that generate exomarkers that can be analysed ex vivo by mass spectrometry to assess levels of reactive species within mitochondria in vivo. We have used one of these compounds, MitoB, to infer the levels of mitochondrial hydrogen peroxide within flies and mice. Here we describe the development of MitoB and expand on this example to discuss how better probes and exomarkers can be developed. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn. [-]
Publicado en
Biochimica et Biophysica Acta (BBA)-General Subjects, 2014, vol. 1840, no 2Derechos de acceso
Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.
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