Evaluation of biogenic amines in fish sauce by derivatization with 3,5-dinitrobenzoyl chloride and micellar liquid chromatography
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Otros documentos de la autoría: Chin-Chen, Mei Liang; Carda-Broch, Samuel; Vives-Peris, Vicente; Rambla Alegre, María; Esteve-Romero, Josep; Marco Peiró, Sergio
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Título
Evaluation of biogenic amines in fish sauce by derivatization with 3,5-dinitrobenzoyl chloride and micellar liquid chromatographyAutoría
Fecha de publicación
2013-02Editor
ElsevierCita bibliográfica
CHIN-CHEN, M. L., et al. Evaluation of biogenic amines in fish sauce by derivatization with 3, 5-dinitrobenzoyl chloride and micellar liquid chromatography. Journal of Food Composition and Analysis, 2013, 29.1: 32-36.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://www.sciencedirect.com/science/article/pii/S0889157512001585Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
A simple, selective and sensitive method to quantify the biogenic amines cadaverine, 2-phenylethylamine, histamine and spermidine has been developed. The analytes were derivatized with 3,5-dinitrobenzoyl chloride and ... [+]
A simple, selective and sensitive method to quantify the biogenic amines cadaverine, 2-phenylethylamine, histamine and spermidine has been developed. The analytes were derivatized with 3,5-dinitrobenzoyl chloride and separated by micellar liquid chromatography. This is a practical technique for the selective determination and quantification of biogenic amines in fish sauce. Optimization of chromatographic conditions was made by an interpretative model, and the separation conditions were: C18 column (125 mm × 4.6 mm, 5 μm particle size), UV detection set at 260 nm, and a mobile phase of 0.15 mol L−1 sodium dodecyl sulfate (SDS), pH 7. Validation was performed following the United States Food and Drug Administration (FDA) guidelines using spiked samples. Under these conditions, validation parameters were: linearity (0.5–500 μg mL−1, r2 > 0.9990), limits of detection (in the 158–375 ng mL−1 range); intra and inter-day precision (relative standard deviation < 3.2% and 4.2%) and accuracy (in the range of 88.6–103.7% and 94.2–101.5%), respectively, and variations were lower than 4%. The proposed method was successfully applied to the monitorization of biogenic amines formation in unsalted and salted fish sauce samples. The suggested methodology was found useful in routine analysis of biogenic amines in fish sauce. [-]
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Journal of Food Composition and Analysis Volume 29, Issue 1, February 2013Derechos de acceso
Copyright © 2014 Elsevier B.V.
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