Fast methodology for the reliable determination of nonylphenol in water samples by minimal labeling isotope dilution mass spectrometry
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Other documents of the author: Fabregat-Cabello, Neus; Castillo Tirado, Ángel; Sancho, Juan V; González, Florenci; Roig-Navarro, Antoni F.
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comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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Fast methodology for the reliable determination of nonylphenol in water samples by minimal labeling isotope dilution mass spectrometryAuthor (s)
Date
2013-08Publisher
ElsevierBibliographic citation
FABREGAT CABELLO, Neus, et al. Fast methodology for the reliable determination of nonylphenol in water samples by minimal labeling isotope dilution mass spectrometry. Journal of Chromatography A, 2013, 1301: 19-26.Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S0021967313007838Version
info:eu-repo/semantics/submittedVersionSubject
Abstract
In this work we have developed and validated an accurate and fast methodology for the determination of 4-nonylphenol (technical mixture) in complex matrix water samples by UHPLC–ESI-MS/MS. The procedure is based on ... [+]
In this work we have developed and validated an accurate and fast methodology for the determination of 4-nonylphenol (technical mixture) in complex matrix water samples by UHPLC–ESI-MS/MS. The procedure is based on isotope dilution mass spectrometry (IDMS) in combination with isotope pattern deconvolution (IPD), which provides the concentration of the analyte directly from the spiked sample without requiring any methodological calibration graph. To avoid any possible isotopic effect during the analytical procedure the in-house synthesized 13C1-4-(3,6-dimethyl-3-heptyl)phenol was used as labeled compound. This proposed surrogate was able to compensate the matrix effect even from wastewater samples. A SPE pre-concentration step together with exhaustive efforts to avoid contamination were included to reach the signal-to-noise ratio necessary to detect the endogenous concentrations present in environmental samples. Calculations were performed acquiring only three transitions, achieving limits of detection lower than 100 ng/g for all water matrix assayed. Recoveries within 83–108% and coefficients of variation ranging from 1.5% to 9% were obtained. On the contrary a considerable overestimation was obtained with the most usual classical calibration procedure using 4-n-nonylphenol as internal standard, demonstrating the suitability of the minimal labeling approach. [-]
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Journal of Chromatography A Volume 1301, 2 August 2013Rights
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