High efficiency joint CZE determination of sugars and acids in vegetables and fruits
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Other documents of the author: Valcárcel, Mercedes; Roselló, Salvador; Cebolla-Cornejo, Jaime; Herrero Martínez, José Manuel; Nuez Viñals, Fernando
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comunitat-uji-handle2:10234/2508
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http://dx.doi.org/10.1002/elps.201100640 |
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Title
High efficiency joint CZE determination of sugars and acids in vegetables and fruitsAuthor (s)
Date
2012-08Publisher
Wiley-VCH Verlag BerlinISSN
1522-2683Bibliographic citation
ELECTROPHORESIS. 2012, Special Issue: Advanced Food Analysis and Foodomics. Volume 33, Issue 15Type
info:eu-repo/semantics/articlePublisher version
http://onlinelibrary.wiley.com/doi/10.1002/elps.201100640/abstractSubject
Abstract
In this work, an improved CE method for the medium-throughput determination of main
organic acids (oxalate, malate, citrate), the amino acid glutamate and the sugars fructose,
glucose and sucrose in several food ... [+]
In this work, an improved CE method for the medium-throughput determination of main
organic acids (oxalate, malate, citrate), the amino acid glutamate and the sugars fructose,
glucose and sucrose in several food matrices is described. These compounds have been
identified as key components in the taste intensity of fruit and vegetable crops. Using a running
buffer with 20 mM 2,6-pyridine dicarboxylic acid pH 12.1 and 0.1% hexadimethrine
bromide, replacing it every 5 h to avoid pH decrease, and optimizing capillary conditioning
between runs with 58 mM SDS during 2 min at 20 psi, it is possible to effectively quantify
these compounds while increasing medium throughput repeatability. This procedure resolves
problems such as increases in migration time and reduction of resolution between
problematic peaks (malate/citrate and fructose/glucose) detected in a previous method.
The new procedure even considerably reduced time analysis down to 12 min. Under optimal
conditions, a large number of injections (200) could be administered without any
disturbances in the same capillary. The reliability of the proposed method was further
investigated with several food matrix samples, including tomato, pepper, muskmelon,
winter squash, and orange. This method is recommended for routine analysis of large
number of samples typical of production quality systems or plant breeding programs. [-]
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© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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