Direct injection of plasma samples and micellar chromatography of procainamide and its metabolite n-acetylprocainamide
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Altres documents de l'autoria: Esteve-Romero, Josep; Martinavarro Domínguez, Adrià; Marcos Tomás, José V.; Ochoa Aranda, Enrique; Rambla Alegre, María
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Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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http://dx.doi.org/10.1365/s10337-009-1467-3 |
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Títol
Direct injection of plasma samples and micellar chromatography of procainamide and its metabolite n-acetylprocainamideAutoria
Data de publicació
2010Editor
Vieweg VerlagISSN
0009-5893Cita bibliogràfica
Chromatographia (2010), 71, 3-4, p. 273-277Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://link.springer.com/article/10.1365/s10337-009-1467-3Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Micellar liquid chromatography was employed for the monitoring of procainamide and its metabolite N-acetylprocainamide using a C18 column. Sodium dodecyl sulphate (SDS) and modifier concentrations were optimised in ... [+]
Micellar liquid chromatography was employed for the monitoring of procainamide and its metabolite N-acetylprocainamide using a C18 column. Sodium dodecyl sulphate (SDS) and modifier concentrations were optimised in order to obtain minimum analysis time, maximum sensitivity and good resolution. The optimum chromatographic conditions were as follows: flow rate 1 mL min−1, injection volume 20 μL, temperature at 25 °C, respectively. The mobile phase consisted of 0.05 M SDS-1% (v/v) butanol–phosphate buffer (10 mM, pH 7, 0.9%, w/v) NaCl using a detection wavelength at 280 nm. Validation studies were carried out according to the ICH guideline and included the determination of calibration curves (r2 > 0.999), intra- and inter-day precisions (CV < 3.9%), robustness and interference studies, respectively. The recoveries in spiked serum samples were adjusted. Finally, the optimized method was applied to serum samples of patients treated with antiarrhythmics, and the results were compared with those given by a reference method where a good correlation was obtained. [-]
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