Application of a liquid chromatographic procedure for the analysis of penicillin antibiotics in biological fluids and pharmaceutical formulations using sodium dodecyl sulphate/propanol mobile phases a.
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Otros documentos de la autoría: Esteve-Romero, Josep; Carda-Broch, Samuel; Rambla Alegre, María
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Application of a liquid chromatographic procedure for the analysis of penicillin antibiotics in biological fluids and pharmaceutical formulations using sodium dodecyl sulphate/propanol mobile phases a.Fecha de publicación
2011-07Editor
© 2010 ElsevierISSN
0021-9673Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://www.sciencedirect.com/science/article/pii/S0021967310016912Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
A direct injection liquid chromatography procedure was developed for the simultaneous determination of four penicillin antibiotics (amoxicillin, ampicillin, cloxacillin and dicloxacillin) in pharmaceutical formulations ... [+]
A direct injection liquid chromatography procedure was developed for the simultaneous determination of four penicillin antibiotics (amoxicillin, ampicillin, cloxacillin and dicloxacillin) in pharmaceutical formulations and physiological fluids (urine) using hybrid micellar mobile phases. These antimicrobials are used to treat gastrointestinal and systemic infections. The four penicillins were analysed using a Zorbax C18 reversed-phase column and detected at 210 nm. These antibiotics were separated by an interpretive optimisation procedure based on the accurate description of the retention and shape of the chromatographic peaks. Antibiotics were eluted in less than 16 min with no interference by the urine protein band or endogenous compounds using the mobile phase 0.11 M sodium dodecyl sulphate–6% propanol–0.01 M NaH2PO4 buffered at pH 3. The method was validated according to the Food and Drug Administration guideline, including analytical parameters such as linearity (R2 > 0.993), intra- and inter-day precisions (RSD, %: 0.1–4.4 and 1.2–5.9, respectively), and robustness for the four compounds. This method is sensitive enough for the routine analysis of penicillins at therapeutic urine levels, with limits of detection in the 1.5–15 ng mL−1 range and limits of quantification of 50 ng mL−1. Recoveries in a micellar medium and a spiked urine matrix were in the 92.4–108.2% and 96–110% ranges, respectively. Finally, the method was successfully applied to determine these antibiotics in urine samples and pharmaceutical formulations. [-]
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Journal of Chromatography A (July 2011) vol. 1218, no. 30, 4972-4981Derechos de acceso
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info:eu-repo/semantics/restrictedAccess
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