Rapid and sensitive determination of nicotine in formulations and biological fluid using micellar liquid chromatography with electrochemical detection
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Otros documentos de la autoría: Chin-Chen, Mei Liang; Rambla Alegre, María; Durgbanshi, Abhilasha; Bose, Devasish; Esteve-Romero, Josep
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http://dx.doi.org/10.1016/j.jchromb.2010.07.010 |
Metadatos
Título
Rapid and sensitive determination of nicotine in formulations and biological fluid using micellar liquid chromatography with electrochemical detectionAutoría
Fecha de publicación
2010Editor
ElsevierISSN
15700232Cita bibliográfica
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 878, 26, p. 2397-2402Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Nicotine can be determined in pharmaceuticals and biological fluids by micellar liquid chromatography (MLC) using a C18 column, a mobile phase containing sodium dodecyl sulphate 0.15M-6% (v/v) pentanol-NaH<sub>2</su ... [+]
Nicotine can be determined in pharmaceuticals and biological fluids by micellar liquid chromatography (MLC) using a C18 column, a mobile phase containing sodium dodecyl sulphate 0.15M-6% (v/v) pentanol-NaH<sub>2</sub>PO<sub>4</sub> 0.01M (pH 6)-KCl 0.001M, with electrochemical detection at 0.8V. In the optimization step, the influence of the modifiers propanol, butanol and pentanol, and the voltage has been studied. With the proposed method the analysis time is below than 8min, linearity better than 0.999, limits of detection and quantification (ng/ml) was 4 and 12 respectively, repeatability and intermediate precision below 1.8%, and all these parameters are adequate for the quantification of nicotine in chewing gum, dermal patches, tobacco and serum samples either by a pharmacologist, pathologist or toxicologist. © 2010 Elsevier B.V. [-]
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