Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals
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Otros documentos de la autoría: Alves da Silva Pimentel, Bruna Natalia; De Annunzio, Sarah Raquel; Assis, Marcelo de; Barbugli, Paula; Longo, Elson; Vergani, Carlos Eduardo
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Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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Título
Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystalsAutoría
Fecha de publicación
2023ISSN
2296-4185Cita bibliográfica
Pimentel BNAS, De Annunzio SR, Assis M, Barbugli PA, Longo E and Vergani CE (2023), Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals. Front. Bioeng. Biotechnol. 11:1215438. doi: 10.3389/fbioe.2023.1215438Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Silver tungstate (α-Ag2WO4), silver molybdate (β-Ag2MoO4), and silver vanadate
(α-AgVO3) microcrystals have shown interesting antimicrobial properties.
However, their biocompatibility is not yet fully understood. ... [+]
Silver tungstate (α-Ag2WO4), silver molybdate (β-Ag2MoO4), and silver vanadate
(α-AgVO3) microcrystals have shown interesting antimicrobial properties.
However, their biocompatibility is not yet fully understood. Cytotoxicity and
the inflammatory response of silver-containing microcrystals were analyzed in
THP-1 and THP-1 differentiated as macrophage-like cells, with the alamarBlue™
assay, flow cytometry, confocal microscopy, and ELISA. The present investigation
also evaluated redox signaling and the production of cytokines (TNFα, IL-1β, IL-6,
and IL-8) and matrix metalloproteinases (MMP-8 and -9). The results showed that
α-AgVO3 (3.9 μg/mL) did not affect cell viability (p > 0.05). α-Ag2WO4 (7.81 μg/mL),
β-Ag2MoO4 (15.62 μg/mL), and α-AgVO3 (15.62 μg/mL) slightly decreased cell
viability (p ≤ 0.003). All silver-containing microcrystals induced the production of
O2
− and this effect was mitigated by Reactive Oxygen Species (ROS) scavenger and
N-acetylcysteine (NAC). TNFα, IL-6 and IL-1β were not detected in THP-1 cells,
while their production was either lower (p ≤ 0.0321) or similar to the control group
(p ≥ 0.1048) for macrophage-like cells. The production of IL-8 by both cellular
phenotypes was similar to the control group (p ≥ 0.3570). The release of MMP-8
was not detected in any condition in THP-1 cells. Although MMP-9 was released
by THP-1 cells exposed to α-AgVO3 (3.9 μg/mL), no significant difference was
found with control (p = 0.7). Regarding macrophage-like cells, the release of
MMP-8 and -9 decreased in the presence of all microcrystals (p ≤ 0.010). Overall,
the present work shows a promising biocompatibility profile of, α-Ag2WO4, βAg2MoO4, and α-AgVO3 microcrystals. [-]
Publicado en
Frontiers in Bioengineering and Biotechnology 11:1215438Entidad financiadora
São Paulo Research Foundation (FAPESP) | FAPESP | European Union-Next Generation
Código del proyecto o subvención
2013/07296-2 (CDMF) | 2018/01677-8 | 2022/08487-5 | MGS/2021/21 (UP 2021-021)
Derechos de acceso
info:eu-repo/semantics/openAccess
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