High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
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Otros documentos de la autoría: Reula, Ana; Pitarch-Fabregat, Javier; MILARA, JAVIER; Cortijo, Julio; Mata Roig, Manuel; Milian Medina, Lara; Armengot, Miguel
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/36080
comunitat-uji-handle3:10234/36082
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INVESTIGACIONMetadatos
Título
High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and TemperatureAutoría
Fecha de publicación
2021-07-20Editor
MDPICita bibliográfica
Reula A, Pitarch-Fabregat J, Milara J, Cortijo J, Mata-Roig M, Milian L, Armengot M. High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature. Diagnostics (Basel). 2021 Jul 20;11(7):1301. doi: 10.3390/diagnostics11071301. PMID: 34359383; PMCID: PMC8305583.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Primary ciliary dyskinesia (PCD) is a rare disease resulting from a defect in ciliary function that generates, among other issues, chronic upper and lower respiratory tract infections. European guidelines recommend ... [+]
Primary ciliary dyskinesia (PCD) is a rare disease resulting from a defect in ciliary function that generates, among other issues, chronic upper and lower respiratory tract infections. European guidelines recommend studying ciliary function (pattern (CBP) and frequency (CBF)), together with characteristic clinical symptoms, as one of the definitive tests. However, there is no “gold standard”. The present study aims to use high-speed video microscopy to describe how CBF and CBP alter over time and at different temperatures to reduce the error rate in the diagnosis of PCD. Samples of nasal epithelium from 27 healthy volunteers were studied to assess CBF and CBP at 0, 3, 24, 48, and 72 h, at room temperature and 4 °C. It was observed that CBF increased while CBP became dyskinetic, both at room temperature and at 4 °C, as time passed, especially after 3 h. In order to preserve all ciliary function parameters and to perform a reliable analysis to improve the diagnostic process of PCD, analysis should be performed within the first 3 h of sample collection, preferably in reference centers. [-]
Publicado en
Diagnostics 2021, 11Entidad financiadora
European Regional Development Fund (ERDF) | Instituto de Salud Carlos III | Spanish Government | Generalitat Valenciana
Código del proyecto o subvención
FIS PI16/01315 | FIS PI19/00949 | CIBERES (CB06 / 06/0027) | Prometheus 2017/023 / UV | FIS PI17 / 02158 | JR18 / 00050 | SAF2017-82913-R
Derechos de acceso
© 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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- MED_Articles [646]