The effect of calcium–magnesium mixtures in sol–gel coatings on bone tissue regeneration
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Otros documentos de la autoría: Cerqueira, Andreia; García-Arnáez, Iñaki; Muriach, Maria; Azkargorta, Mikel; Elortza, Felix; Izquierdo, Raúl; Romero-Gavilán, Francisco J; GURRUCHAGA, MARILO; Suay, Julio; Goñi, Isabel
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Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7034
comunitat-uji-handle3:10234/8619
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INVESTIGACIONMetadatos
Título
The effect of calcium–magnesium mixtures in sol–gel coatings on bone tissue regenerationAutoría
Fecha de publicación
2022Editor
Royal Society of ChemistryISSN
2047-4830; 2047-4849Cita bibliográfica
Cerqueira A, García-Arnáez I, Muriach M, Azkargorta M, Elortza F, Izquierdo R, Romero-Gavilán F, Gurruchaga M, Suay J, Goñi I. The effect of calcium-magnesium mixtures in sol-gel coatings on bone tissue regeneration. Biomater. Sci., 2022, 10(19), 5634-5647. DOI: 10.1039/d2bm00742h.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://pubs.rsc.org/en/content/articlehtml/2022/bm/d2bm00742hVersión
info:eu-repo/semantics/submittedVersionPalabras clave / Materias
Resumen
Calcium and magnesium are two elements essential for bone structure and metabolism. However, their synergistic or competitive effects on bone regeneration are often overlooked during biomaterial development. We examined ... [+]
Calcium and magnesium are two elements essential for bone structure and metabolism. However, their synergistic or competitive effects on bone regeneration are often overlooked during biomaterial development. We examined the interactions between Ca and Mg in sol–gel coatings doped with mixtures of CaCl2 (0.5%) and MgCl2 (0.5, 1, and 1.5%). After physicochemical characterisation, the materials were incubated in vitro with MC3T3-E1 osteoblastic cells and RAW264.7 macrophages, and the protein adsorption was analysed using nLC-MS/MS. The incorporation of the ions did not lead to the formation of crystalline structures and did not affect the sol–gel network cross-linking. The release of the ions did not cause cytotoxic effects at any tested concentration. The proteomic analysis showed that adding the Ca and Mg ions elevated the adsorption of proteins associated with inflammatory response regulation (e.g., ALBU, CLUS, HPT, HPTR, A1AG1 and A1AG2) but decreased the adsorption of immunoglobulins. The CaMg coatings had reduced affinity to proteins associated with coagulation (e.g., FA9, FA10, FA11, FA12) but increased the adsorption of proteins involved in cell adhesion (DSG1, DESP, FBLN1, ZA2G). In vitro assays revealed that the cellular response was affected by changing the concentration of Mg. Moreover, our results show that these differences reflect the changes in the concentrations of both ions in the mix but are not a simple additive effect. [-]
Publicado en
Biomaterials Science, 2022, vol. 10, no 19Entidad financiadora
Ministerio de Ciencia, Innovación y Universidades | Generalitat Valenciana | Universitat Jaume I | Basque Government
Identificador de la entidad financiadora
http://dx.doi.org/10.13039/501100011033
Código del proyecto o subvención
MICIU/ICTI2017-2020/PID2020-113092RB-C21 | RTC-2017-6147-1 | GRISOLIAP/2018/091 | APOSTD/2020/036 | PROMETEO/2020/069 | GACUJI/2021/14 | PRE_2017_2_0044
Título del proyecto o subvención
Desarrollo de recubrimientos osteoinductores y obtención de biomarcadores de regeneración ósea
Derechos de acceso
Copyright © The Royal Society of Chemistry
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info:eu-repo/semantics/openAccess
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/openAccess
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