Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic study
Impacto
Scholar |
Otros documentos de la autoría: Cerqueira, Andreia; García-Arnáez, Iñaki; Romero-Gavilán, Francisco J; Azkargorta, Mikel; Elortza, Felix; Martín de Llanos, José Javier; Carda, Carmen; GURRUCHAGA, MARILO; Goñi, Isabel; Suay, Julio
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7034
comunitat-uji-handle3:10234/8619
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INVESTIGACIONMetadatos
Título
Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic studyAutoría
Fecha de publicación
2022Editor
ElsevierISSN
2772-9508Cita bibliográfica
A. Cerqueira, I. García-Arnáez, F. Romero-Gavilán, M. Azkargorta, F. Elortza, J. J. Martín de Llanos, C. Carda, M. Gurruchaga, I. Goñi, J. Suay, Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic study, Biomater. Adv., (2022), 212826, https://doi.org/10.1016/j.bioadv.2022.212826.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.sciencedirect.com/science/article/pii/S2772950822001030Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
The cell–biomaterial interface is highly complex; thousands of molecules and many processes participate in its formation. Growing demand for improved biomaterials has highlighted the need to understand the structure ... [+]
The cell–biomaterial interface is highly complex; thousands of molecules and many processes participate in its formation. Growing demand for improved biomaterials has highlighted the need to understand the structure and functions of this interface. Proteomic methods offer a viable alternative to the traditional in vitro techniques for analyzing such systems. Magnesium is a promoter of cell adhesion and osteogenesis. Here, we used the LC-MS/MS to compare the protein expression profiles of human osteoblasts (HOb) exposed to sol-gel coatings without (MT) and with Mg (MT1.5Mg) for 1, 3, and 7 days. PANTHER, DAVID, and IPA databases were employed for protein identification and data analysis. Confocal microscopy and gene expression analysis were used for further characterization. Exposure to MT1.5Mg increased the HOb cell area and the expression of SP7, RUNX2, IBP3, COL3A1, MXRA8, and FBN1 genes. Proteomic analysis showed that MT1.5Mg affected the early osteoblast maturation (PI3/AKT, mTOR, ERK/MAPK), insulin metabolism, cell adhesion (integrin, FAK, actin cytoskeleton regulation) and oxidative stress pathways. Thus, the effects of Mg on cell adhesion and osteogenesis are rather complex, affecting several pathways rather than single processes. Our analysis also confirms the potential of proteomics in biomaterial characterization, showing a good correlation with in vitro results. [-]
Publicado en
Biomaterials Advances, 2022Entidad financiadora
Ministerio de Ciencia, Innovación y Universidades | Generalitat Valenciana | Universitat Jaume I | Basque Government
Código del proyecto o subvención
MICIU/ICTI2017-2020/PID2020-113092RB-C21 | GRISOLIAP/2018/091 | APOSTD/2020/036 | PROMETEO/2020/069 | UJI-B2021-25 | PRE_2017_2_0044
Derechos de acceso
info:eu-repo/semantics/openAccess
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