IRS1 expression in hippocampus is age-dependent and is required for mature spine maintenance and neuritogenesis
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Otros documentos de la autoría: Sánchez-Sarasúa, Sandra; Meseguer Beltrán, Maria; García Díaz, Cristina; Beltrán-Bretones, Maria Teresa; ElMlili, Nisrin; Sánchez-Pérez, Ana María
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INVESTIGACIONMetadatos
Título
IRS1 expression in hippocampus is age-dependent and is required for mature spine maintenance and neuritogenesisAutoría
Fecha de publicación
2022-01Editor
ElsevierISSN
1044-7431Cita bibliográfica
Sánchez-Sarasúa S, Meseguer-Beltrán M, García-Díaz C, Beltrán-Bretones MT, ElMlili N, Sánchez-Pérez AM. IRS1 expression in hippocampus is age-dependent and is required for mature spine maintenance and neuritogenesis. Mol Cell Neurosci. 2021 Dec 20;118:103693. doi: 10.1016/j.mcn.2021.103693Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.sciencedirect.com/science/article/pii/S1044743121001068?via%3DihubVersión
info:eu-repo/semantics/acceptedVersionPalabras clave / Materias
Resumen
Insulin and insulin-like growth factor type I (IGF-1) play prominent roles in brain activity throughout the lifespan. Insulin/IGF1 signaling starts with the activation of the intracellular insulin receptor substrates ... [+]
Insulin and insulin-like growth factor type I (IGF-1) play prominent roles in brain activity throughout the lifespan. Insulin/IGF1 signaling starts with the activation of the intracellular insulin receptor substrates (IRS). In this work, we performed a comparative study of IRS1 and IRS2, together with the IGF1 (IGF1R) and insulin (IR) receptor expression in the hippocampus and prefrontal cortex during development. We found that IRS1 and IRS2 expression is prominent during development and declines in the aged hippocampus, contrary to IR, which increases in adulthood and aging. In contrast, IGF1R expression is unaffected by age. Expression patterns are similar in the prefrontal cortex. Neurite development occurs postnatally in the rodent hippocampus and cortex, and it declines in the mature and aged brain and is influenced by trophic factors. In our previous work, we demonstrated that knockdown of IRS1 by shRNA impairs learning and reduces synaptic plasticity in a rat model, as measured by synaptophysin puncta in axons. In this study, we report that shIRS1 alters spine maturation in adult hilar hippocampal neurons. Lastly, to understand the role of IRS1 in neuronal neurite tree, we transfect shIRS1 into primary neuronal cultures and observed that shIRS1 reduced neurite branching and neurite length.
Our results demonstrate that IRS1/2 and insulin/IGF1 receptors display different age-dependent expression profiles and that IRS1 is required for spine maturation, demonstrating a novel role for IRS1 in synaptic plasticity. [-]
Publicado en
Molecular and Cellular Neuroscience, 2022, vol. 118Entidad financiadora
Universitat Jaume I | Conselleria de Educació, Cultura i Sport | Conselleria d'Innovació, Universitats, Ciència i Societat Digital
Código del proyecto o subvención
UJI-B2018-01 | AICO/2015/042 | ACIF/2016/250
Derechos de acceso
Copyright © Elsevier B.V.
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info:eu-repo/semantics/openAccess
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info:eu-repo/semantics/openAccess
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