Identification of new, very long-chain polyunsaturated fatty acids in fish by gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization
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Otros documentos de la autoría: Serrano, Roque; Navarro, Juan Carlos; Portoles, Tania; Sales Martínez, Carlos; Beltran Arandes, Joaquin; Monroig, Oscar; Hernandez, Felix
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https://doi.org/10.1007/s00216-020-03062-0 |
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Título
Identification of new, very long-chain polyunsaturated fatty acids in fish by gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionizationAutoría
Fecha de publicación
2021-2Editor
SpringerISSN
1618-2642; 1618-2650Cita bibliográfica
Serrano, R., Navarro, J.C., Portolés, T. et al. Identification of new, very long-chain polyunsaturated fatty acids in fish by gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization. Analiticla and Bioanalitical Chemistry, 413, 1039–1046 (2021). https://doi.org/10.1007/s00216-020-03062-0Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://link.springer.com/article/10.1007/s00216-020-03062-0Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
The characterization of very long-chain (>C24) polyunsaturated fatty acids (VLC-PUFAs), which are essential in the vision, neural function, and reproduction of vertebrates, is challenging because of the lack of reference ... [+]
The characterization of very long-chain (>C24) polyunsaturated fatty acids (VLC-PUFAs), which are essential in the vision, neural function, and reproduction of vertebrates, is challenging because of the lack of reference standards and their very low concentrations in certain lipid classes. In this research, we have developed a new methodology for VLC-PUFA identification based on gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with an atmospheric pressure chemical ionization source (GC-APCI-QTOF MS). The mass accuracy attainable with the innovative QTOF instrument, together with the soft ionization of the APCI source, provides valuable information on the intact molecule, traditionally lost with electron ionization sources due to the extensive fragmentation suffered. We have identified, for the first time, VLC-PUFAs with chains up to 44 carbons in eyes, brain, and gonads of gilthead sea bream, a commercially important fish in the Mediterranean. The added value of ion mobility-mass spectrometry (IMS), recently developed in combination with GC-QTOF MS, and the contribution of the collisional cross section (CCS) parameter in the characterization of novel VLC-PUFAs (for which reference standards are not available) have been also evaluated. The methodology developed has allowed assessing qualitative differences between farmed and wild fish, and opens new perspectives in a still scarcely known field of research. [-]
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Analytical and Bioanalytical Chemistry, 2021Proyecto de investigación
Programa Estatal de Investigacion, Desarrollo e Innovacion orientada a los retos de la sociedad (Ministry of Economy and Competitiveness): AGL2013-40986-R; Ramon y Cajal Program from the Ministry of Economy and Competitiveness, Spain: RYC-201722525; Generalitat Valenciana 2019/040; University Jaume I: UJI-B201808Derechos de acceso
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