Exploring the Origin of Amidase Substrate Promiscuity in CALB by a Computational Approach
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Otros documentos de la autoría: Galmés, Miquel À; García-Junceda, Eduardo; Świderek, Katarzyna; Moliner, Vicent
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Título
Exploring the Origin of Amidase Substrate Promiscuity in CALB by a Computational ApproachFecha de publicación
2020Editor
American Chemical SocietyISSN
2155-5435Cita bibliográfica
Miquel À Galmés, Eduardo García-Junceda, Katarzyna Świderek, and Vicent Moliner.Exploring the Origin of Amidase Substrate Promiscuity in CALB by a Computational Approach ACS Catalysis 2020 10 (3), 1938-1946 DOI: 10.1021/acscatal.9b04002Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://pubs.acs.org/doi/full/10.1021/acscatal.9b04002Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Enzyme promiscuity attracts the interest of the industrial and academic sectors because of its application in the design of biocatalysts. The amidase activity of Candida antarctica lipase B (CALB) on two different ... [+]
Enzyme promiscuity attracts the interest of the industrial and academic sectors because of its application in the design of biocatalysts. The amidase activity of Candida antarctica lipase B (CALB) on two different substrates has been studied by theoretical quantum mechanics/molecular mechanics methods, supported by experimental kinetic measurements. The aim of the study is to understand the substrate promiscuity of CALB in this secondary reaction and the origin of its promiscuous catalytic activity. The computational results predict activation free energies in very good agreement with the kinetic data and confirm that the activity of CALB as an amidase, despite depending on the features of the amide substrate, is dictated by the electrostatic effects of the protein. The protein polarizes and activates the substrate as well as stabilizes the transition state, thus enhancing the rate constant. Our results can provide guides for future designs of biocatalysts based on electrostatic arguments. [-]
Publicado en
ACS Catalysis, 2019, vol. 10, no 3Proyecto de investigación
Spanish Ministerio de Ciencia, Innovación y Universidades: Grant PGC2018-094852-B-C21; Spanish Ministerio de Economía y Competitividad: Grant MAT2015-65184-C2-2-R; Universitat Jaume I: project UJI·B2017- 31; National Institutes of Health: Ref no. NIH R01 GM065368; MINECO for a Juan de la Cierva—Incorporación: ref IJCI-2016-27503; Universitat Jaume I: PREDOC/2017/23Derechos de acceso
This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
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info:eu-repo/semantics/openAccess
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