Parallel reaction pathways and noncovalent intermediates in thymidylate synthase revealed by experimental and computational tools
Ver/ Abrir
Impacto
Scholar |
Otros documentos de la autoría: Kholodar, Svetlana; Ghosh, Ananda K; Świderek, Katarzyna; Moliner, Vicent; Kohen, Amnon
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
comunitat-uji-handle4:
INVESTIGACIONMetadatos
Título
Parallel reaction pathways and noncovalent intermediates in thymidylate synthase revealed by experimental and computational toolsFecha de publicación
2018Editor
National Academy of SciencesISSN
0027-8424; 1091-6490Cita bibliográfica
KHOLODAR, Svetlana A., et al. Parallel reaction pathways and noncovalent intermediates in thymidylate synthase revealed by experimental and computational tools. Proceedings of the National Academy of Sciences, 2018, vol. 115, no 41, p. 10311-10314.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.pnas.org/content/115/41/10311Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Thymidylate synthase was one of the most studied enzymes due
to its critical role in molecular pathogenesis of cancer. Nevertheless, many atomistic details of its chemical mechanism remain
unknown or debated, thereby ... [+]
Thymidylate synthase was one of the most studied enzymes due
to its critical role in molecular pathogenesis of cancer. Nevertheless, many atomistic details of its chemical mechanism remain
unknown or debated, thereby imposing limits on design of novel
mechanism-based anticancer therapeutics. Here, we report unprecedented isolation and characterization of a previously proposed intact noncovalent bisubstrate intermediate formed in the
reaction catalyzed by thymidylate synthase. Free-energy surfaces
of the bisubstrate intermediates interconversions computed with
quantum mechanics/molecular mechanics (QM/MM) methods and
experimental assessment of the corresponding kinetics indicate
that the species is the most abundant productive intermediate
along the reaction coordinate, whereas accumulation of the covalent bisubstrate species largely occurs in a parallel nonproductive pathway. Our findings not only substantiate relevance of the
previously proposed noncovalent intermediate but also support
potential implications of the overstabilized covalent intermediate
in drug design targeting DNA biosynthesis. [-]
Publicado en
PNAS, October 9, 2018, vol. 115, no. 41Proyecto de investigación
R01 GM65368 ; CTQ2015-66223-C2 and UJI·B2017-31.Derechos de acceso
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- QFA_Articles [811]
El ítem tiene asociados los siguientes ficheros de licencia: