In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography
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Other documents of the author: Molenaar, Sam D.; Sleutels, Tom; Pereira, Joao; Iorio, Matteo; Borsje, Casper; Zamudio, Julian A.; Fabregat-Santiago, Francisco; Buisman, Cees; Ter Heijne, Annemiek
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comunitat-uji-handle2:10234/160292
comunitat-uji-handle3:10234/160293
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Title
In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence TomographyAuthor (s)
Date
2018-07-11Publisher
WileyISSN
1864-5631; 1864-564XBibliographic citation
MOLENAAR, Sam D., et al. In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography. ChemSusChem, 2018, vol. 11, no 13, p. 2171-2178Type
info:eu-repo/semantics/articlePublisher version
https://onlinelibrary.wiley.com/doi/full/10.1002/cssc.201800589Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and ... [+]
Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real‐time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well‐established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated to growth yields between 38 and 42 mgurn:x-wiley:18645631:media:cssc201800589:cssc201800589-math-0001 gurn:x-wiley:18645631:media:cssc201800589:cssc201800589-math-0002 −1 at an anode potential of −0.35 V versus Ag/AgCl. Time‐lapse observations of an experimental run performed in duplicate show high reproducibility in obtained microbial growth yield by the developed method. As such, we identify OCT as a powerful tool for conducting in‐depth characterizations of microbial growth dynamics in BESs. Additionally, the presented platform allows concomitant application of this method with various optical and electrochemical techniques. [-]
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ChemSusChem, 2018, vol. 11, no 13Rights
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