Quantification and identification of steroidal hormones in sole (solea senegalensis) by ultra high performance liquid chromatography coupled to tandem mass spectrometry
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/158176
comunitat-uji-handle2:10234/71324
comunitat-uji-handle3:10234/98504
comunitat-uji-handle4:
TFG-TFMEste recurso está restringido
Metadatos
Título
Quantification and identification of steroidal hormones in sole (solea senegalensis) by ultra high performance liquid chromatography coupled to tandem mass spectrometryAutoría
Tutor/Supervisor; Universidad.Departamento
Serrano Gallego, Roque; Universitat Jaume I. Departament de Química Física i Analítica; Beltrán Iturat, EduardoFecha de publicación
2017Editor
Universitat Jaume IResumen
The study of steroid hormones is essential to understand reproduction processes, for
example sex differentiation, puberty, gonad maduration or others reproductive cycles.
In biological matrices steroidal hormones ... [+]
The study of steroid hormones is essential to understand reproduction processes, for
example sex differentiation, puberty, gonad maduration or others reproductive cycles.
In biological matrices steroidal hormones are present at low levels and thus, a robust
and sensitive analytical method is required. Ultra High Performance Liquid
chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) is a suitable
technique for the simultaneous determination of sexual hormones. This methodology
was developed for the analysis of 14 of the most relevant fish steroids, including
androgens (4-androstenedione (A4), 11β-hydroxyandrostenedione (11βOHA), 11-
ketoandrostenedione (11KA), testosterone (T), 5α-dihydrotestosterone (DHT), 11-
ketotestosterone (11KT)), estrogens (estrone (E1), estradiol (E2)) and corticosteroids
(cortisol (F), 11-deoxycortisol (S)), and progestogens (pregnenolone (Preg),
progesterone (P), 17α,20β-dihydroxy-4-pregnen-3-one (17, 20β-P),
17α,20β,21,trihydroxy-4-pregnen-3-one (20β-S)). The method was developed and
validated for different types of samples of the flatfish Senegalese sole. Sample
treatment for plasma consists in addition of acetonitrile for precipitation the proteins
and a subsequent clean-up by solid-phase extraction (SPE). Urine was passed through
the SPE cartridges. Hormones of interest were determined using a triple quadrupole
analyzer with a rapid chromatography separation in reversed phase (6 min). Free and
total hormones were analyzed, total hormones included a sum of free and conjugated
hormones. For determination the total hormones a hydrolysis with β-glucoronidase
was performed. The method was validated by means of recovery experiments,
obtaining adequate results of accuracy and precision. Lowest level validated was 0.1
ng·mL-1 and the Limit of detection (LOD) was in the range of pg·mL-1. The acquisition of
three MS/MS transitions for each compound allowed the unequivocal confirmation of
positive samples. [-]
Palabras clave / Materias
Descripción
Treball Final de Grau en Química. Codi: QU0943. Curs acadèmic: 2016/2017.
Tipo de documento
info:eu-repo/semantics/bachelorThesisDerechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
Aparece en las colecciones
- Grau en Química [265]