Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel
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Otros documentos de la autoría: Kalu, Nnanya; Alcaraz, Antonio; Yamini, Goli; Mombem Abolfath, Sanaz; Lucas, Laura; Kenney, Clare; Aguilella, Vicente; Nestorovich, Ekaterina M.
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INVESTIGACIONMetadatos
Título
Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 ChannelAutoría
Fecha de publicación
2017-11Editor
WileyCita bibliográfica
KALU, Nnanya; ALCARAZ GONZÁLEZ, Antonio; YAMINI, Goli; MOMBEM ABOLFATH, Sanaz; LUCAS, Laura; KENNEY, Clare; AGUILELLA FERNÁNDEZ, Vicente; NESTOROVICH, Ekaterina M. Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel. FEBS Letters (2017), v. 591, issue 21, p. 3481-3492Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12866/abstract;jsessionid=6 ...Versión
info:eu-repo/semantics/submittedVersionPalabras clave / Materias
Resumen
Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ ... [+]
Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ by many intracellularly active bacterial exotoxins, including the anthrax toxin. Here we investigate the role of pH on single-channel properties of the anthrax toxin channel, PA63. Using conductance and current noise analysis, blocker binding, ion selectivity, and PEG partitioning measurements, we show that the channel exists in two different open states (‘maximum’ and ‘main’) at pH 5.5, while only a maximum conductance state is detected at pH 5.5. We describe two substantially distinct patterns of PA63 conductance dependence on KCl concentration uncovered at pH 6.5 and 4.5. [-]
Publicado en
FEBS Letters (2017), v. 591, issue 21, p. 3481-3492Proyecto de investigación
The project was financially supported by: 1) NIAID of the NIH under award number 1R15AI099897-01A1; 2) The Catholic University startup funds (to Ekaterina Nestorovich); 3) Spanish Government (FIS2013-40473-P and FIS2016-75257-P AEI/FEDER), 4) Universitat Jaume I (P1.1B2015-28); 5) American Dissertation Fellowship from AAUWDerechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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