Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel
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Other documents of the author: Kalu, Nnanya; Alcaraz, Antonio; Yamini, Goli; Mombem Abolfath, Sanaz; Lucas, Laura; Kenney, Clare; Aguilella, Vicente; Nestorovich, Ekaterina M.
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Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2507
comunitat-uji-handle3:10234/6973
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Title
Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 ChannelAuthor (s)
Date
2017-11Publisher
WileyBibliographic citation
KALU, Nnanya; ALCARAZ GONZÁLEZ, Antonio; YAMINI, Goli; MOMBEM ABOLFATH, Sanaz; LUCAS, Laura; KENNEY, Clare; AGUILELLA FERNÁNDEZ, Vicente; NESTOROVICH, Ekaterina M. Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel. FEBS Letters (2017), v. 591, issue 21, p. 3481-3492Type
info:eu-repo/semantics/articlePublisher version
http://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12866/abstract;jsessionid=6 ...Version
info:eu-repo/semantics/submittedVersionSubject
Abstract
Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ ... [+]
Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ by many intracellularly active bacterial exotoxins, including the anthrax toxin. Here we investigate the role of pH on single-channel properties of the anthrax toxin channel, PA63. Using conductance and current noise analysis, blocker binding, ion selectivity, and PEG partitioning measurements, we show that the channel exists in two different open states (‘maximum’ and ‘main’) at pH 5.5, while only a maximum conductance state is detected at pH 5.5. We describe two substantially distinct patterns of PA63 conductance dependence on KCl concentration uncovered at pH 6.5 and 4.5. [-]
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FEBS Letters (2017), v. 591, issue 21, p. 3481-3492Investigation project
The project was financially supported by: 1) NIAID of the NIH under award number 1R15AI099897-01A1; 2) The Catholic University startup funds (to Ekaterina Nestorovich); 3) Spanish Government (FIS2013-40473-P and FIS2016-75257-P AEI/FEDER), 4) Universitat Jaume I (P1.1B2015-28); 5) American Dissertation Fellowship from AAUWRights
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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