Determination of selected endogenous anabolic androgenic steroids and ratios in urine by ultra high performance liquid chromatography tandem mass spectrometry and isotope pattern deconvolution
Impacto
Scholar |
Otros documentos de la autoría: Pitarch-Motellón, Jorge; Sancho, Juan V; Ibáñez, Maria; Pozo, Óscar J.; Roig-Navarro, Antoni F.
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/33596
comunitat-uji-handle3:10234/33597
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INVESTIGACIONMetadatos
Título
Determination of selected endogenous anabolic androgenic steroids and ratios in urine by ultra high performance liquid chromatography tandem mass spectrometry and isotope pattern deconvolutionAutoría
Fecha de publicación
2017-09Editor
ElsevierCita bibliográfica
PITARCH-MOTELLÓN, Jorge, et al. Determination of selected endogenous anabolic androgenic steroids and ratios in urine by ultra high performance liquid chromatography tandem mass spectrometry and isotope pattern deconvolution. Journal of Chromatography A, 2017, vol. 1515, p. 172-178.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://www.sciencedirect.com/science/article/pii/S0021967317311482Versión
info:eu-repo/semantics/draftPalabras clave / Materias
Resumen
An isotope dilution mass spectrometry (IDMS) method for the determination of selected endogenous anabolic androgenic steroids (EAAS) in urine by UHPLC–MS/MS has been developed using the isotope pattern deconvolution ... [+]
An isotope dilution mass spectrometry (IDMS) method for the determination of selected endogenous anabolic androgenic steroids (EAAS) in urine by UHPLC–MS/MS has been developed using the isotope pattern deconvolution (IPD) mathematical tool. The method has been successfully validated for testosterone, epitestosterone, androsterone and etiocholanolone, employing their respective deuterated analogs using two certified reference materials (CRM). Accuracy was evaluated as recovery of the certified values and ranged from 75% to 108%. Precision was assessed in intraday (n = 5) and interday (n = 4) experiments, with RSDs below 5% and 10% respectively. The method was also found suitable for real urine samples, with limits of detection (LOD) and quantification (LOQ) below the normal urinary levels. The developed method meets the requirements established by the World Anti-Doping Agency for the selected steroids for Athlete Biological Passport (ABP) measurements, except in the case of androsterone, which is currently under study. [-]
Proyecto de investigación
Generalitat Valenciana (Research group of excellence Prometeo II 2014/023 and Collaborative Research on Environment and Food Safety ISIC/2012/016) ; University Jaume I (PB1-1B2013-55)Derechos de acceso
© 2017 Published by Elsevier B.V.
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info:eu-repo/semantics/openAccess
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
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