Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECTin Guinea Pig: Role of Bitter Taste Agonists
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Otros documentos de la autoría: Ortiz, José L.; Ortiz, Amparo; MILARA, JAVIER; Armengot, Miguel; Sanz, Celia; Compañ, Desamparados; Morcillo, Esteban; Cortijo, Julio
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Título
Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECTin Guinea Pig: Role of Bitter Taste AgonistsAutoría
Fecha de publicación
2016Editor
Public Library of ScienceISSN
1932-6203Cita bibliográfica
Ortiz JL, Ortiz A, Milara J, Armengot M, Sanz C, Compañ D, et al. (2016) Evaluation of Mucociliary Clearance by Three Dimension MicroCT-SPECT in Guinea Pig: Role of Bitter Taste Agonists. PLoS ONE 11(10): e0164399. doi:10.1371/journal.pone.0164399Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164399Resumen
Different image techniques have been used to analyze mucociliary clearance (MCC) in
humans, but current small animal MCC analysis using in vivo imaging has not been well
defined. Bitter taste receptor (T2R) agonists ... [+]
Different image techniques have been used to analyze mucociliary clearance (MCC) in
humans, but current small animal MCC analysis using in vivo imaging has not been well
defined. Bitter taste receptor (T2R) agonists increase ciliary beat frequency (CBF) and
cause bronchodilation but their effects in vivo are not well understood. This work analyzes
in vivo nasal and bronchial MCC in guinea pig animals using three dimension (3D) microCT-SPECT
images and evaluates the effect of T2R agonists. Intranasal macroaggreggates
of albumin-Technetium 99 metastable (MAA-Tc99m) and lung nebulized Tc99m albumin
nanocolloids were used to analyze the effect of T2R agonists on nasal and bronchial MCC
respectively, using 3D micro-CT-SPECT in guinea pig. MAA-Tc99m showed a nasal mucociliary
transport rate of 0.36 mm/min that was increased in presence of T2R agonist to 0.66
mm/min. Tc99m albumin nanocolloids were homogeneously distributed in the lung of
guinea pig and cleared with time-dependence through the bronchi and trachea of guinea
pig. T2R agonist increased bronchial MCC of Tc99m albumin nanocolloids. T2R agonists
increased CBF in human nasal ciliated cells in vitro and induced bronchodilation in human
bronchi ex vivo. In summary, T2R agonists increase MCC in vivo as assessed by 3D microCT-SPECT
analysis. [-]
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PLoS ONE, October 10, 2016Derechos de acceso
info:eu-repo/semantics/openAccess
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