Metabolomic approaches for olive oil quality discrimination by gas chromatography coupled to mass spectrometry
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Metabolomic approaches for olive oil quality discrimination by gas chromatography coupled to mass spectrometryAutoría
Tutor/Supervisor
Beltrán Arandes, JoaquimTutor/Supervisor; Universidad.Departamento
Universitat Jaume I. Departament de Química Física i AnalíticaFecha de publicación
2016-06-24Editor
Universitat Jaume IResumen
In this work, a gas chromatography coupled to mass spectrometry (GC-EI-Q(MS))
has been used for markers identification for a metabolomics approach. It has been
carried out in order to create a statistical model to ... [+]
In this work, a gas chromatography coupled to mass spectrometry (GC-EI-Q(MS))
has been used for markers identification for a metabolomics approach. It has been
carried out in order to create a statistical model to perform a correct classification
between different quality olive oils (extra, virgin and lampante). A dynamic headspace
purge and trap (DHS-P&T) was used to the extraction of volatile organic compounds
(VOCs) responsible for olive oil flavours and they were eluted from the SPE cartridges
with a mixture of hexane:diethyl ether (50/50, v/v). The methodology is based on the
application of P&T extraction followed by gas chromatography coupled to mass
spectrometry in full-spectrum acquision mode. The data acquired by this equipment was
pre-treated by a new program called PARAFAC. After that these acquired data was
normalized and the differentiation between the different groups of olive oil has been
carried out with a multivariate analysis using Ez-Info. This methodology was applied to
process 47 olive oil samples (11 extra, 19 virgin, 17 lampante) in order to create and
check the classification model. The different models were performed with 37 samples.
Finally, the whole developed method was correctly validated by the other 10 samples
which were kept for the validation. Four of the seven evaluated model achieved around
a 60% of correct classification. And with one of the studied models was obtained a
classification of 90%. So with that result, it can be said that with this methodology and
with these markers the PANEL TEST could be substituted. [-]
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Treball Final de Grau en Química. Codi: QU0943. Curs: 2015/2016
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