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Excess white noise to probe transport mechanisms in a membrane channel
dc.contributor.author | Queralt-Martín, María | |
dc.contributor.author | López Peris, María Lidón | |
dc.contributor.author | Alcaraz, Antonio | |
dc.date.accessioned | 2016-05-11T14:32:57Z | |
dc.date.available | 2016-05-11T14:32:57Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1539-3755 | |
dc.identifier.issn | 1550-2376 | |
dc.identifier.uri | http://hdl.handle.net/10234/159561 | |
dc.description.abstract | Current fluctuation analysis has been successfully used over the years to investigate the physical properties of different systems. Here, we perform single-channel time-resolved current experiments in a protein channel to evaluate the different transport mechanisms governing the channel function. Using different salts of monovalent and divalent cations in a wide range of concentrations and applied potentials, we analyze current fluctuations focusing on the voltage dependence of the additional white noise that appears in the low-frequency range of the spectra. We demonstrate that the channel displays two characteristic transport regimes: at low salt concentrations (10 mM to 1 M) ion permeation is controlled by the protein fixed charges that induce accumulation or exclusion of ions to preserve local electroneutrality. At high salt concentrations (>1 M) adsorption processes associated to the binding of cations to the channel charges regulate the transport properties. | ca_CA |
dc.description.sponsorShip | Spanish Ministry of Economy and Competitiveness (MINECO) FIS2013-40473-P Fundacio Caixa Castello-Bancaixa P1-1B2012-03 | ca_CA |
dc.format.extent | 5 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | American Physical Society | ca_CA |
dc.relation.isPartOf | PHYSICAL REVIEW E 91, 062704 (2015) | ca_CA |
dc.rights | ©2015 American Physical Society | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | ompf-porin | ca_CA |
dc.subject | escherichia-coli | ca_CA |
dc.subject | crystal-structures | ca_CA |
dc.subject | ion selectivity | ca_CA |
dc.subject | 1/f noise | ca_CA |
dc.subject | dynamics | ca_CA |
dc.subject | protein | ca_CA |
dc.title | Excess white noise to probe transport mechanisms in a membrane channel | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1103/PhysRevE.91.062704 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | http://journals.aps.org/pre/abstract/10.1103/PhysRevE.91.062704 | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion |
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